| Induction of inflammatory cell accumulation by TM-N49 and promutoxin, two novel phospholipase A(2). | |
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MedLine Citation:
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PMID: 20538012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Local inflammation is a prominent characteristic of snakebite wound. Snake venom phospholipase A(2)s (PLA(2)s) are one of the main components which contribute to accumulation of inflammatory cells. We have isolated TM-N49 and promutoxin from Protobothrops mucrosquamatus venom and investigated their ability in induction of cell accumulation by using an in vivo mouse model. The results showed that both TM-N49 and promutoxin are potent stimuli for induction of neutrophil, lymphocyte, macrophage and eosinophil accumulation in the mouse peritoneum. The TM-N49- and promutoxin-induced inflammatory cell accumulation was inhibited by pretreatment of animals with cyproheptadine, terfenadine and Ginkgolide B, indicating that histamine and PAF is likely to contribute to the cells accumulation. Pre-injection of antibodies against adhesion molecules ICAM-1, CD18, CD11a and L-selectin showed that ICAM-1 is a key adhesion molecule of TM-N49- and promutoxin-induced lymphocyte, macrophage and eosinophil accumulation; CD18 and CD11a plays an important role in the migration of neutrophils, eosinophils and macrophages; and L-selectin is involved in the neutrophil and eosinophil accumulation. In conclusion, induction of inflammatory cell accumulation by TM-N49 and promutoxin confirms that group II PLA(2)s is pivotal stimulus for cell infiltration, through which they participate in the formation of snakebite inflammation. |
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Authors:
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Ji-Fu Wei; Xiao-long Wei; Qiu-Yu Chen; Shao-Heng He |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-09 |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 56 ISSN: 1879-3150 ISO Abbreviation: Toxicon Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-12-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: England |
Other Details:
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Languages: eng Pagination: 580-8 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Clinical Experiment Center, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents / pharmacology Antibodies, Monoclonal / pharmacology Cell Movement / drug effects Crotalid Venoms / enzymology* Eosinophils / drug effects, immunology Group II Phospholipases A2 / isolation & purification, toxicity* Immunity, Cellular / drug effects Inflammation / chemically induced* Lymphocytes / drug effects, immunology Macrophages / drug effects, immunology Male Mice Mice, Inbred BALB C Neutrophils / drug effects, immunology Reptilian Proteins / isolation & purification, toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/Antibodies, Monoclonal; 0/Crotalid Venoms; 0/Reptilian Proteins; EC 3.1.1.4/Group II Phospholipases A2; EC 3.1.1.4/TM-N49 protein, Protobothrops mucrosquamatus |
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