Document Detail

Induction of inflammatory cell accumulation by TM-N49 and promutoxin, two novel phospholipase A(2).
MedLine Citation:
PMID:  20538012     Owner:  NLM     Status:  MEDLINE    
Local inflammation is a prominent characteristic of snakebite wound. Snake venom phospholipase A(2)s (PLA(2)s) are one of the main components which contribute to accumulation of inflammatory cells. We have isolated TM-N49 and promutoxin from Protobothrops mucrosquamatus venom and investigated their ability in induction of cell accumulation by using an in vivo mouse model. The results showed that both TM-N49 and promutoxin are potent stimuli for induction of neutrophil, lymphocyte, macrophage and eosinophil accumulation in the mouse peritoneum. The TM-N49- and promutoxin-induced inflammatory cell accumulation was inhibited by pretreatment of animals with cyproheptadine, terfenadine and Ginkgolide B, indicating that histamine and PAF is likely to contribute to the cells accumulation. Pre-injection of antibodies against adhesion molecules ICAM-1, CD18, CD11a and L-selectin showed that ICAM-1 is a key adhesion molecule of TM-N49- and promutoxin-induced lymphocyte, macrophage and eosinophil accumulation; CD18 and CD11a plays an important role in the migration of neutrophils, eosinophils and macrophages; and L-selectin is involved in the neutrophil and eosinophil accumulation. In conclusion, induction of inflammatory cell accumulation by TM-N49 and promutoxin confirms that group II PLA(2)s is pivotal stimulus for cell infiltration, through which they participate in the formation of snakebite inflammation.
Ji-Fu Wei; Xiao-long Wei; Qiu-Yu Chen; Shao-Heng He
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-09
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  56     ISSN:  1879-3150     ISO Abbreviation:  Toxicon     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  580-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Clinical Experiment Center, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology
Antibodies, Monoclonal / pharmacology
Cell Movement / drug effects
Crotalid Venoms / enzymology*
Eosinophils / drug effects,  immunology
Group II Phospholipases A2 / isolation & purification,  toxicity*
Immunity, Cellular / drug effects
Inflammation / chemically induced*
Lymphocytes / drug effects,  immunology
Macrophages / drug effects,  immunology
Mice, Inbred BALB C
Neutrophils / drug effects,  immunology
Reptilian Proteins / isolation & purification,  toxicity*
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Antibodies, Monoclonal; 0/Crotalid Venoms; 0/Reptilian Proteins; EC II Phospholipases A2; EC protein, Protobothrops mucrosquamatus

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