Document Detail

Induction of HIV-1 replication in latently infected syncytiotrophoblast cells by contact with placental macrophages: role of interleukin-6 and tumor necrosis factor-alpha.
MedLine Citation:
PMID:  11798466     Owner:  NLM     Status:  MEDLINE    
The syncytiotrophoblast (ST) layer of the human placenta has an important role in limiting transplacental viral spread from mother to fetus. Although certain strains of human immunodeficiency virus type 1 (HIV-1) may enter ST cells, the trophoblast does not exhibit permissiveness for HIV-1. The present study tested the possibility that placental macrophages might induce replication of HIV-1 carried in ST cells and, further, that infected ST cells would be capable of transmitting virus into neighboring macrophages. For this purpose, we investigated HIV-1 replication in ST cells grown alone or cocultured with uninfected placental macrophages. The macrophage-tropic Ba-L strain of HIV-1, capable of entering ST cells, was used throughout our studies. We demonstrated that interactions between ST cells and macrophages activated HIV-1 from latency and induced its replication in ST cells. After having become permissive for viral replication, ST cells delivered HIV-1 to the cocultured macrophages, as evidenced by detection of virus-specific antigens in these cells. The stimulatory effect of coculture on HIV-1 gene expression in ST cells was mediated by marked tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) release from macrophages, an effect caused by contact between the different placental cells. Results of this study suggest an interactive role for the ST layer and placental macrophages in the dissemination of HIV-1 among placental tissue. Data reported here may also explain why macrophage-tropic HIV-1 strains are transmitted preferentially during pregnancy.
A Bácsi; E Csoma; Z Beck; I Andirkó; J Kónya; L Gergely; F D Tóth
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research     Volume:  21     ISSN:  1079-9907     ISO Abbreviation:  J. Interferon Cytokine Res.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2002-01-18     Completed Date:  2002-03-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9507088     Medline TA:  J Interferon Cytokine Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1079-88     Citation Subset:  IM    
Institute of Microbiology and Tumor Virus Research Group, Hungarian Academy of Sciences, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
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MeSH Terms
Antibodies / pharmacology
Cells, Cultured
Coculture Techniques
Cytokines / physiology*
Gene Products, tat / metabolism
HIV Core Protein p24 / metabolism
HIV-1 / growth & development*,  metabolism
Interleukin-6 / antagonists & inhibitors,  immunology,  physiology
Macrophages / immunology*
Microscopy, Fluorescence
Placenta / immunology*
Trophoblasts / virology*
Tumor Necrosis Factor-alpha / antagonists & inhibitors,  immunology,  physiology
Virus Replication
tat Gene Products, Human Immunodeficiency Virus
Reg. No./Substance:
0/Antibodies; 0/Cytokines; 0/Gene Products, tat; 0/HIV Core Protein p24; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; 0/tat Gene Products, Human Immunodeficiency Virus

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