Document Detail


Induction of G1 arrest and differentiation in MDA-MB-231 breast cancer cell by boehmeriasin A, a novel compound from plant.
MedLine Citation:
PMID:  16445628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Boehmeriasin A is a new phenanthroquinolizidine alkaloid recently isolated from the Boehmeria siamensis Craib (Urticaceae). In vitro biological activity assay demonstrated that this novel compound has wide-range, strong antitumor activity. This study is aimed to determine the effects of boehmeriasin A on breast cancer cell (MDA-MB-231 cell line). Proliferation assay and fluorescence activated cell sorter (FACS) showed that cell growth inhibition and G1 phase arrest of cell cycle were caused by boehmeriasin A. The concentrations resulting in total and 50% growth inhibition are 0.007 and 0.0035 microg/mL, respectively. Exposed in 0.007 microg/mL boehmeriasin A for 12 h, the G1 phase cell percent increased from 44.8% pre-drug treatment to 66.3%. Consistent with G1 arrest and cell growth inhibition, cyclin E2 and cyclin D1 messenger RNA expression in the cell was down-regulated with drug treatment. Then, few apoptotic cells were detected, and most other cells underwent differentiation, which is characterized by specific changes in cell morphology, lots of lipid droplet accumulation, and increasing expression of adipocyte differentiation-related protein. The result first demonstrates that boehmeriasin A potently inhibits the proliferation of breast cancer cell MDA-MB-231 via the G1 phase cell cycle arrest and differentiation induction, and as such, may be considered as candidate chemotherapeutic and/or chemopreventive agent for breast cancer.
Authors:
J Yan; D Luo; Y Luo; X Gao; G Zhang
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  International journal of gynecological cancer : official journal of the International Gynecological Cancer Society     Volume:  16     ISSN:  1048-891X     ISO Abbreviation:  Int. J. Gynecol. Cancer     Publication Date:    2006 Jan-Feb
Date Detail:
Created Date:  2006-01-31     Completed Date:  2006-05-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9111626     Medline TA:  Int J Gynecol Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-70     Citation Subset:  IM    
Affiliation:
Institute of materia medica, Di Ao Pharmaceutical Group, Chengdu 610041, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Base Sequence
Boehmeria*
Breast Neoplasms / pathology
Cell Cycle / drug effects
Cell Differentiation / drug effects*
Cell Division / drug effects
Cell Line, Tumor / cytology,  drug effects
Cyclin-Dependent Kinase 2 / drug effects,  metabolism*
Down-Regulation
Female
G1 Phase / drug effects*,  physiology
Humans
Immunoblotting
Molecular Sequence Data
Plant Structures*
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 2.7.11.22/Cyclin-Dependent Kinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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