Document Detail


Induction of Cr(VI) reduction activity in an Anoxybacillus strain under heat stress: a biochemical and proteomic study.
MedLine Citation:
PMID:  22448780     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A bacterial strain, designated as TSB-6, was isolated from the sediments of a Tantloi (India) hot spring at 65°C. The strain showed 98% 16S rRNA gene sequence similarity with Anoxybacillus kualawohkensis strain KW12 and was found to grow optimally at 37°C. However, growing cells, cell suspensions and cell-free extracts from 65°C cultures showed higher Cr(VI)-reduction activities when assayed at either 37°C or 65°C than those obtained from 37°C cultures. On fractionation of extracts from cells grown at 65°C, the chromate reductase activity assayed at 65°C was found mostly in the soluble fraction. When log phase cells growing at 37°C were shifted to 65°C, the stressed cells produced larger quantities of reactive oxygen species (ROS). Consequently, growth of the cells was retarded but specific Cr(VI)-reduction activity increased. 2D gel electrophoresis followed by MALDI-TOF MS/MS identified the proteins whose expression level changed as a result of heat stress. The upregulated set included proteins involved in cellular metabolism of sugar, nucleotide, amino acids, lipids and vitamins, oxidoreductase activity and protein folding. The downregulated proteins are also involved in cellular metabolism, DNA binding and environmental signal processing. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Sangita Chowdhury Paul; Preeti Jain; Jyotirmoy Mitra; Sangita Dutta; Pamela Bhattacharya; Bijay Bal; Debasish Bhattacharyya; Sujoy Das Gupta; Subrata Pal
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-26
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Department of Life Science and Biotechnology, Jadavpur University, Kolkata, 700 032.
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