| The induction of bystander mutagenic effects in vivo by alpha-particle irradiation in whole Arabidopsis thaliana plants. | |
| | |
MedLine Citation:
|
PMID: 20681789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Our previous studies demonstrated distant/abscopal bystander effects in A. thaliana seeds and embryos; the postembryonic development of bystander tissues, such as root hair differentiation, primary root elongation, lateral root initiation and survival, were inhibited significantly by localized irradiation with microbeam protons and low-energy ions. In the present study, we further investigated radiation-induced bystander mutagenic effects in vivo in A. thaliana plants using homologous recombination (HR) and the expression level of the HR-related AtRAD54 gene as mutagenic end points. We found that alpha-particle irradiation of distal primary roots of young seedlings resulted in a significant increase in the frequency of HR in the aerial plants; the increased induction of HR occurred in every true leaf over the course of rosette development. Moreover, we also found that localized alpha-particle irradiation of roots induced a short-term up-regulated expression of the HR-related AtRAD54 gene in the nonirradiated aerial plants. These results suggested the existence of bystander mutagenic effects in vivo in plants. Treatment with the ROS scavenger DMSO dramatically reduced the effects of localized root irradiation on the induction of HR and expression of the AtRAD54 gene in bystander tissues, suggesting that ROS play a critical role in mediating the bystander mutagenic effects in plants. |
| | |
Authors:
|
Fanghua Li; Ping Liu; Ting Wang; Po Bian; Yuejin Wu; Lijun Wu; Zengliang Yu |
Related Documents
:
|
3575799 - Everything is a carcinogen? 16314139 - Genotoxic and mutagenic activity of environmental air samples from different rural, urb... 7265289 - Design and testing of a biocontainment system for chemical carcinogens. 19957249 - Mutagenic and antimutagenic effects of hexane extract of some astragalus species grown ... 8528869 - Stability of ondansetron hydrochloride injection in various beverages. 6356339 - Biological monitoring of occupational exposure to mutagenic chemicals in the rubber ind... 14871739 - Growth, shoot phenology and physiology of diverse seed sources of black spruce: ii. 23-... 20436669 - Epigenetic variation in mangrove plants occurring in contrasting natural environment. 20597289 - Positive adjacency effects mediated by seed disperser birds in pine plantations. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Radiation research Volume: 174 ISSN: 1938-5404 ISO Abbreviation: Radiat. Res. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-04 Completed Date: 2010-10-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0401245 Medline TA: Radiat Res Country: United States |
Other Details:
|
Languages: eng Pagination: 228-37 Citation Subset: IM; S |
Affiliation:
|
Key Laboratory of Ion Beam Bio-engineering, Institute of Plasma Physics of Chinese Academy of Sciences, Hefei 230031, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alpha Particles* Arabidopsis / genetics, physiology*, radiation effects Arabidopsis Proteins / genetics, radiation effects Bystander Effect / physiology* DNA-Binding Proteins / genetics, radiation effects Gene Expression Regulation, Plant Mutagenesis Mutagenicity Tests Plant Roots / physiology, radiation effects Recombination, Genetic / radiation effects Seedling / physiology, radiation effects Up-Regulation |
| Chemical | |
Reg. No./Substance:
|
0/Arabidopsis Proteins; 0/DNA-Binding Proteins; 0/RAD54 protein, Arabidopsis |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Investigation of adaptive responses in bystander cells in 3D cultures containing tritium-labeled and...
Next Document: Yields of Clustered DNA Damage Induced by Charged-Particle Radiations of Similar Kinetic Energy per ...