| Induction of apoptosis in the human Leukemic U937 cell line by Kaempferia parviflora Wall.ex.Baker extract and effects of paclitaxel and camptothecin. | |
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MedLine Citation:
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PMID: 20192599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Kaempferia parviflora Wall.ex.Baker is a Thai medicinal herb that has high antioxidant and anti-inflammatory activities. Apoptotic effects of the herbal extract alone and in combination with chemotherapeutic drugs, paclitaxel and camptothecin, were here studied in the human promonocytic leukemic U937 cell line. K. parviflora extract suppressed cell proliferation and decreased cell viability in a dose- and time-dependent manner as assessed using the trypan blue exclusion assay. Staining of extract-treated cells with propidium iodide and examination under a fluorescence microscope showed condensed nuclei and apoptotic bodies. Mitochondrial transmembrane potential (MTP) decreased after treatment and the number of cells with decreased MTP also increased. Furthermore, activation of caspase-3 was found in herbal extract-treated cells. When the extract was combined with paclitaxel, an additive effect on U937 cell apoptosis was obtained, whereas camptothecin exerted an antagonistic effect. |
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Authors:
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Ratana Banjerdpongchai; Yupa Chanwikruy; Viboon Rattanapanone; Bungorn Sripanidkulchai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Asian Pacific journal of cancer prevention : APJCP Volume: 10 ISSN: 1513-7368 ISO Abbreviation: Asian Pac. J. Cancer Prev. Publication Date: 2009 |
Date Detail:
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Created Date: 2010-03-02 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101130625 Medline TA: Asian Pac J Cancer Prev Country: Thailand |
Other Details:
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Languages: eng Pagination: 1137-40 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. ratana@chiangmai.ac.th |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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pharmacology* Apoptosis / drug effects*, physiology Camptothecin / pharmacology* Caspase 3 / metabolism Cell Line, Tumor Dose-Response Relationship, Drug Flow Cytometry Humans Microscopy, Fluorescence Paclitaxel / pharmacology* Phytotherapy Plant Extracts / pharmacology* Rhizome Statistics, Nonparametric U937 Cells / drug effects*, metabolism Zingiberaceae* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Plant Extracts; 33069-62-4/Paclitaxel; 7689-03-4/Camptothecin; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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