Document Detail

Induction of Apoptosis of SW480 Human Colon Cancer Cells by (-)-Epicatechin Isolated from Bulnesia sarmienti.
MedLine Citation:
PMID:  23225437     Owner:  NLM     Status:  In-Data-Review    
MATERIALS AND METHODS: Cells were treated with different concentrations (0, 25, 50, and 100 μmol/ml) of (-)-epicatechin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, 40,6-diamidine-20-phenylindole dihydrochloride (DAPI) staining, colony-forming assay, DNA fragmentation analysis, reverse transcription-polymerase chain reaction (RT-PCR), annexin V-fluorescein isothiocyanate (FITC) staining, and immunoblot analyses were then carried out.
RESULTS: (-)-Epicatechin was found to have cytotoxic activity, and cells treated with this compound had fragmented nuclei, fragmented DNA, and underwent apoptosis. mRNA and protein expression levels of BCL2-associated X protein (BAX) and p53 were up-regulated and those of B-cell lymphoma-2 (BCL2) were down-regulated, while p21 mRNA levels were significantly increased in cells treated with (-)-epicatechin in a concentration-dependent manner.
CONCLUSION: (-)-Epicatechin from B. Sarmienti inhibited colon cancer cell growth and induced apoptosis.
Daeik Kim; Mohammad Lalmoddin Mollah; Kilsoo Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  32     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  5353-61     Citation Subset:  IM    
DVM, College of Veterinary Medicine, Kyungpook National University, #1370 Sangyeok-dong, Buk-gu, Daegu, 702-701, Republic of Korea.
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