Document Detail


Induction of Apoptosis of SW480 Human Colon Cancer Cells by (-)-Epicatechin Isolated from Bulnesia sarmienti.
MedLine Citation:
PMID:  23225437     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
MATERIALS AND METHODS: Cells were treated with different concentrations (0, 25, 50, and 100 μmol/ml) of (-)-epicatechin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, 40,6-diamidine-20-phenylindole dihydrochloride (DAPI) staining, colony-forming assay, DNA fragmentation analysis, reverse transcription-polymerase chain reaction (RT-PCR), annexin V-fluorescein isothiocyanate (FITC) staining, and immunoblot analyses were then carried out.
RESULTS: (-)-Epicatechin was found to have cytotoxic activity, and cells treated with this compound had fragmented nuclei, fragmented DNA, and underwent apoptosis. mRNA and protein expression levels of BCL2-associated X protein (BAX) and p53 were up-regulated and those of B-cell lymphoma-2 (BCL2) were down-regulated, while p21 mRNA levels were significantly increased in cells treated with (-)-epicatechin in a concentration-dependent manner.
CONCLUSION: (-)-Epicatechin from B. Sarmienti inhibited colon cancer cell growth and induced apoptosis.
Authors:
Daeik Kim; Mohammad Lalmoddin Mollah; Kilsoo Kim
Related Documents :
24122997 - Aberrant expression of ezh2 is associated with pathological findings and p53 alteration.
24523387 - Oxalate up-regulates expression of il-2rβ and activates il-2r signaling in hk-2 cells,...
24676907 - Cardiomyocyte differentiation induced in cardiac progenitor cells by cardiac fibroblast...
23719947 - Analyzing the circulating micrornas in exosomes/extracellular vesicles from serum or pl...
23485457 - Nfκb and mapk signalling pathways mediate tnfα-induced early growth response gene tra...
22405697 - Thiacremonone, a sulfur compound isolated from garlic, attenuates lipid accumulation pa...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  32     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  5353-61     Citation Subset:  IM    
Affiliation:
DVM, College of Veterinary Medicine, Kyungpook National University, #1370 Sangyeok-dong, Buk-gu, Daegu, 702-701, Republic of Korea. kskim728@knu.ac.kr.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Artonin E Mediates MCL1 Down-regulation and Sensitizes Lung Cancer Cells to Anoikis.
Next Document:  Suppressive effects of cyclophosphamide and gemcitabine on regulatory T-cell induction in vitro.