Document Detail

Inducing proliferation of human amniotic epithelial (HAE) cells for cell therapy.
MedLine Citation:
PMID:  11144969     Owner:  NLM     Status:  MEDLINE    
Probably because amnion is derived from the fetus and is exposed to the maternal immune system, human amniotic epithelial (HAE) cells do not express the HLA-A, -B, -C, or -DR antigens on their surfaces, suggesting that HAE cells do not induce rejection (immune reaction) after allotransplantation. And the amnion, like the placenta, is useless to the mother and child after birth. Therefore, HAE cells or tissues were expected to be suitable for allotransplantation. Because HAE cells produce large amounts of enzymes, amnion transplantation has been carried out in order to correct inborn errors of metabolism by supplementing lysosomal enzyme deficiencies. However, several problems remain before amnion allotransplantation can be accepted as effective. The HAE cell population is limited, because the maximum number of HAE cells obtainable from one donor is about 2 x 10(8) cells, and HAE cells proliferate poorly in in vitro culture. In this study, we aimed at increasing the HAE cell population in vitro. First, we investigated the effect of several cytokines on HAE cell proliferation and found that hepatocyte growth factor (HGF), epidermal growth factor (EGF), and transforming growth factor-beta stimulated it, whereas IL-6 and LIF inhibited it. Second, we investigated the effects of amniotic fluid on HAE cell proliferation and observed that IL-6 in amniotic fluid inhibits it. Then, to inhibit the dying of cells, we attempted to inhibit apoptosis (one mode of cell death). Treatment with caspase III inhibitor increased the cell viability of HAE cells by 20%.
S Terada; K Matsuura; S Enosawa; M Miki; A Hoshika; S Suzuki; N Sakuragawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell transplantation     Volume:  9     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:    2000 Sep-Oct
Date Detail:
Created Date:  2000-12-28     Completed Date:  2001-03-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  701-4     Citation Subset:  IM    
Department of Applied Chemistry & Biotechnology, Faculty of Engineering, Fukui University, Japan.
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MeSH Terms
Amnion / cytology*
Amniotic Fluid / immunology
Apoptosis / drug effects
Cell Culture Techniques / methods
Cell Division
Cell Transplantation / methods*
Cells, Cultured
Cysteine Proteinase Inhibitors / pharmacology
Cytokines / pharmacology
Epidermal Growth Factor / pharmacology
Epithelial Cells / cytology*,  drug effects
Hepatocyte Growth Factor / pharmacology
Interleukin-6 / immunology
Oligopeptides / pharmacology
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/Cytokines; 0/Interleukin-6; 0/Oligopeptides; 0/aspartyl-glutamyl-valyl-aspartal; 62229-50-9/Epidermal Growth Factor; 67256-21-7/Hepatocyte Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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