Document Detail

Inducible transcription factor expression in a cell culture model of apoptosis.
MedLine Citation:
PMID:  10095097     Owner:  NLM     Status:  MEDLINE    
We have developed a model of nerve cell death based on the toxicity of okadaic acid, a compound that triggers apoptosis in PC12 cells via a protein synthesis-dependent mechanism. The cell death process is accompanied by induction of JunB, c-Jun, JunD and Fos proteins. Phosphorylation-specific antibodies were used to demonstrate that c-Jun is phosphorylated at serine 63 and serine 73. Electrophoretic gel mobility shift and pAP1-Luc luciferase assays showed that expression of ITFs is associated with increases in AP-1 binding and in AP-1 transcriptional activity. In addition, dose response and time course studies provided strong correlative evidence that Fos and Jun proteins are involved in the apoptotic death cascades. Thus, this model provides a useful system to investigate the role of inducible transcription factor proteins in apoptosis.
A Woodgate; M Walton; G A MacGibbon; M Dragunow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  66     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-05-13     Completed Date:  1999-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  211-6     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Published by Elsevier Science B.V.
The Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Cyclic AMP Response Element-Binding Protein / genetics,  metabolism
DNA Probes
Enzyme Inhibitors / pharmacology
Gene Expression / drug effects,  genetics
Neurons / chemistry,  cytology*,  physiology*
Okadaic Acid / pharmacology
PC12 Cells
Proto-Oncogene Proteins c-jun / genetics,  metabolism
Transcription Factor AP-1 / genetics*,  metabolism
Reg. No./Substance:
0/Cyclic AMP Response Element-Binding Protein; 0/DNA Probes; 0/Enzyme Inhibitors; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; 78111-17-8/Okadaic Acid

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