Document Detail


Inducible nitric oxide synthase depresses cardiac contractile function in Zucker diabetic fatty rats.
MedLine Citation:
PMID:  17976576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cardiac contractile dysfunction is a common occurrence in type 2 diabetes. The aim was to examine if inducible nitric oxide synthase (iNOS) causes cardiac dysfunction in Zucker diabetic fatty (ZDF) rats, a model of type 2 diabetes. ZDF and Zucker lean control rats (20 week old) were studied at 6 h after recovery from halothane anaesthesia and surgery that involved insertions of catheters into the iliac arteries, iliac veins and the left ventricle via the right carotid artery. Protein expression and activity of iNOS in the hearts were measured by immunostaining and arginine-citrulline conversion assay, respectively. Both groups had similar baseline left ventricular developed pressure and maximum rate of rise of left ventricular pressure (+dP/dt), but heart rate and rate pressure product were lower in the ZDF than control rats. Dobutamine dose-dependently increased left ventricular developed pressure, +dP/dt, heart rate and rate pressure product in both groups, but the responses were less in the diabetic than control rats. The activity and protein expression of iNOS and nitrotyrosine were higher in the hearts of the diabetic than control rats. Selective inhibition of iNOS by 1400 W (N-3-aminomethyl-benzyl-acetamidine) did not alter responses to dobutamine in the control rats, but augmented the effects of dobutamine on left ventricular developed pressure and rate pressure product in the diabetic rats. The results indicate that activation of iNOS contributed to left ventricular contractile dysfunction in the ZDF rats, and this was partially reversed by selective inhibition of the activity of iNOS.
Authors:
Dongzhe Song; Kuo-Hsing Kuo; Reina Yao; Simon R Hutchings; Catherine C Y Pang
Related Documents :
3020526 - Pressor, tachycardic and behavioral excitatory responses in conscious rats following ic...
7095396 - Effects of diltiazem on renovascular-hypertensive and on normotensive rats.
383156 - Age-dependent increase in the formation of prostaglandin i2 by intact and homogenised a...
17044666 - The vasodepressor function of the kidney: further characterization of medullipin and a ...
6716186 - Blood pressure self-monitoring in the workplace.
16197936 - Mercury free operation of the coulter counter multisizer ii sampling stand.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-12
Journal Detail:
Title:  European journal of pharmacology     Volume:  579     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-08     Completed Date:  2008-04-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  253-9     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, Pharmacology and Therapeutics, Faculty of Medicine, The University of British Columbia, 2176 Health Sciences Mall, Vancouver, B.C., Canada V6T 1Z3.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adrenergic beta-Agonists / administration & dosage,  pharmacology
Animals
Diabetes Mellitus, Type 2 / complications*,  physiopathology
Disease Models, Animal
Dobutamine / administration & dosage,  pharmacology
Dose-Response Relationship, Drug
Gene Expression Regulation, Enzymologic
Male
Myocardial Contraction / physiology*
Nitric Oxide Synthase Type II / metabolism*
Rats
Rats, Zucker
Ventricular Function, Left / physiology*
Chemical
Reg. No./Substance:
0/Adrenergic beta-Agonists; 34368-04-2/Dobutamine; EC 1.14.13.39/Nitric Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Silencing of herg gene by shRNA inhibits SH-SY5Y cell growth in vitro and in vivo.
Next Document:  Nobiletin, a citrus flavonoid with neurotrophic action, augments protein kinase A-mediated phosphory...