| Inducible expression of stem cell associated intermediate filament nestin reveals an important role in glioblastoma carcinogenesis. | |
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MedLine Citation:
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PMID: 20669222 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intermediate filament nestin is transiently expressed in neural stem/progenitor cells during the development of central nervous system. Recently, increasing evidence has shown that upregulation of nestin is related to malignancy of several cancers, especially glioblastoma. However, the function of nestin in carcinogenesis remains unclear. In this study, we investigated the role of nestin in glioblastoma carcinogenesis by comparing subclones of rat C6 glioblastoma cells that were either high or low for nestin expression. We found that while nestin expression did not influence the in vitro proliferation of glioblastoma cells, subclones characterized by high levels of nestin formed tumors in vivo at significantly faster rates than subclones with low expression. Importantly, C6 subclones that expressed nestin at low levels in vitro were also found to give rise to tumors highly positive for the protein, suggesting that induction of nestin plays an important role in glioblastoma carcinogenesis. Derivation of nestin positive tumors from nestin negative human U87 glioblastoma cells in immunodeficient mice further confirmed that a switch to positive expression of nestin is fundamental to the course of glioblastoma development. Blocking the expression of nestin in glioblastoma tumors via intratumor injection of shRNA significantly slowed tumor growth and volume. These results demonstrated that nestin plays a crucial role in development of glioblastoma and may potentially be targeted for treatment of the disease. |
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Authors:
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Wen Jing Lu; Feng Lan; Qihua He; Andrew Lee; Chad Z Tang; Lin Dong; Baojin Lan; Xiaowen Ma; Joseph C Wu; Li Shen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-28 |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 128 ISSN: 1097-0215 ISO Abbreviation: Int. J. Cancer Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 343-51 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 UICC. |
Affiliation:
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Department of Cell Biology, Peking University Health Science Center, Beijing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Neoplasms / etiology* Cell Line, Tumor Glioblastoma / chemistry, etiology* Humans Intermediate Filament Proteins / analysis, genetics, physiology* Intermediate Filaments / chemistry* Mice Mice, Inbred BALB C Mice, Nude Nerve Tissue Proteins / analysis, genetics, physiology* RNA, Small Interfering / genetics Rats Stem Cells / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/RNA, Small Interfering; 0/nestin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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