| Induced differentiation and maturation of newborn liver cells into functional hepatic tissue in macroporous alginate scaffolds. | |
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MedLine Citation:
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PMID: 18070820 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present work explores cell cultivation in macroporous alginate scaffolds as a means to reproduce hepatocyte terminal differentiation in vitro. Newborn rat liver cell isolates, consisting of proliferating hepatocytes and progenitors, were seeded at high cell density of 125 x 10(6)/cm(3) within the scaffold and then cultivated for 6 wk in chemically defined medium. Within 3 days, the alginate-seeded cells expressed genes for mature liver enzymes, such as tryptophan oxygenase, secreted a high level of albumin, and performed phase I drug metabolism. The cells formed compacted spheroids, establishing homotypic and heterotypic cell-to-cell interactions. By 6 wk, the spheroids developed into organoids, with an external mature hepatocyte layer covered by a laminin layer encasing inner vimentin-positive cells within a laminin-rich matrix also containing collagen. The hepatocytes presented a distinct apical surface between adjacent cells and a basolateral surface with microvilli facing extracellular matrix deposits. By contrast, viable adherent cells within collagen scaffolds presenting the identical porous structure did not express adult liver enzymes or secrete albumin after 6 wk. This study thus illustrates the benefits of cell cultivation in macroporous alginate scaffolds as an effective promoter for the maturation of newborn liver cells into functional hepatic tissue, capable of maintaining prolonged hepatocellular functions. |
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Authors:
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Mona Dvir-Ginzberg; Tsiona Elkayam; Smadar Cohen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-10 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 22 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-05-02 Completed Date: 2008-05-28 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 1440-9 Citation Subset: IM |
Affiliation:
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Ben-Gurion University of the Negev, Department of Biotechnology Engineering, P.O. 653, Beer-Sheva 84105, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alginates* Animals Animals, Newborn Cell Differentiation Cytochrome P-450 Enzyme System / metabolism Hepatocytes / cytology* Liver / growth & development* Microscopy, Electron, Transmission Rats Rats, Sprague-Dawley Stem Cells / cytology Tissue Engineering / methods* |
| Chemical | |
Reg. No./Substance:
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0/Alginates; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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