Document Detail

Induced pluripotent stem cells generated from adult bone marrow-derived cells of the nonhuman primate (Callithrix jacchus) using a novel quad-cistronic and excisable lentiviral vector.
MedLine Citation:
PMID:  23194452     Owner:  NLM     Status:  MEDLINE    
Regenerative medicine is in need of solid, large animal models as a link between rodents and humans to evaluate the functionality, immunogenicity, and clinical safety of stem cell-derived cell types. The common marmoset (Callithrix jacchus) is an excellent large animal model, genetically close to humans and readily used worldwide in clinical research. Until now, only two groups showed the generation of induced pluripotent stem cells (iPSCs) from the common marmoset using integrating retroviral vectors. Therefore, we reprogrammed bone marrow-derived mesenchymal cells (MSCs) of adult marmosets in the presence of TAV, SB431542, PD0325901, and ascorbic acid via a novel, excisable lentiviral spleen focus-forming virus (SFFV)-driven quad-cistronic vector system (OCT3/4, KLF4, SOX2, C-MYC). Endogenous pluripotency markers like OCT3/4, KLF4, SOX2, C-MYC, LIN28, NANOG, and strong alkaline phosphatase signals were detected. Exogenous genes were silenced and additionally the cassette was removed with a retroviral Gag precursor system. The cell line could be cultured in absence of leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF) and could be successfully differentiated into embryoid bodies and teratomas with presence of all three germ layers. Directed differentiation generated neural progenitors, megakaryocytes, adipocytes, chondrocytes, and osteogenic cells. Thus, all criteria for fully reprogrammed bone marrow-MSCs of a nonhuman primate with a genetically sophisticated construct could be demonstrated. These cells will be a promising tool for future autologous transplantations.
Anastasia Wiedemann; Kathrin Hemmer; Inga Bernemann; Gudrun Göhring; Olena Pogozhykh; Constanca Figueiredo; Silke Glage; Axel Schambach; Jens C Schwamborn; Rainer Blasczyk; Thomas Müller
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular reprogramming     Volume:  14     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-05-23     Revised Date:  2013-09-19    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  485-96     Citation Subset:  IM    
Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
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MeSH Terms
Adult Stem Cells* / cytology,  metabolism
Bone Marrow Cells* / cytology,  metabolism
Gene Expression
Genetic Vectors*
Induced Pluripotent Stem Cells* / cytology,  metabolism
Transcription Factors / biosynthesis*,  genetics
Transduction, Genetic*
Reg. No./Substance:
0/Transcription Factors
Erratum In:
Cell Reprogram. 2013 Aug;15(4):337

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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