| Indoxyl sulphate induces oxidative stress and the expression of osteoblast-specific proteins in vascular smooth muscle cells. | |
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MedLine Citation:
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PMID: 19164326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Previously, we demonstrated that indoxyl sulphate (IS), a uraemic toxin, induced aortic calcification in hypertensive rats. This study aimed to determine if IS induces the production of reactive oxygen species (ROS) and the expression of osteoblast-specific proteins in human aortic smooth muscle cells (HASMCs). METHODS: In order to achieve these goals, HASMCs were incubated with IS. ROS were detected using probes with a fluorescence detector. The expression of alkaline phosphatase (ALP), osteopontin and organic anion transporters (OAT1, OAT3) was studied by western blotting. The expression of core binding factor 1 (Cbfa1), ALP, osteopontin and NADPH oxidases (Nox1, Nox2 and Nox4) was analysed by reverse transcription-polymerase chain reaction (RT-PCR). Knockdown of Nox4 was performed by RNA interference (RNAi). RESULTS: IS induced ROS generation and the expression of Nox4, Cbfa1, ALP and osteopontin in HASMCs. A NADPH oxidase inhibitor and antioxidants inhibited IS-induced ROS production and mRNA expression of Cbfa1 and ALP. Knockdown of Nox4 using small interfering RNA (siRNA) inhibited IS-induced ROS production and mRNA expression of Cbfa1, ALP and osteopontin. OAT3 was expressed in HASMCs. CONCLUSIONS: IS induces ROS generation by upregulating Nox4, and the expression of osteoblast-specific proteins such as Cbfa1, ALP and osteopontin in HASMCs. |
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Authors:
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Gulinuer Muteliefu; Atsushi Enomoto; Ping Jiang; Masahide Takahashi; Toshimitsu Niwa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-22 |
Journal Detail:
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Title: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association Volume: 24 ISSN: 1460-2385 ISO Abbreviation: Nephrol. Dial. Transplant. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-22 Completed Date: 2009-08-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8706402 Medline TA: Nephrol Dial Transplant Country: England |
Other Details:
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Languages: eng Pagination: 2051-8 Citation Subset: IM |
Affiliation:
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Department of Clinical Preventive Medicine, Nagoya University Hospital, Nagoya, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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biosynthesis* Cells, Cultured Core Binding Factor Alpha 1 Subunit / biosynthesis* Humans Indican / pharmacology* Muscle, Smooth, Vascular / cytology, drug effects*, physiology* Osteopontin / biosynthesis* Oxidative Stress / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Core Binding Factor Alpha 1 Subunit; 106441-73-0/Osteopontin; 487-94-5/Indican; EC 3.1.3.1/Alkaline Phosphatase |
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