Document Detail

Indoleamine 2,3-dioxygenase activity in the aqueous humor, iris/ciliary body, and retina of the bovine eye.
MedLine Citation:
PMID:  8224949     Owner:  NLM     Status:  MEDLINE    
The enzyme indoleamine 2,3-dioxygenase (IDO), which uses free oxygen radicals to cleave the pyrrole ring of indoleamines and give kynurenamines, has previously been found in most tissues, but not in the eye. In this study, IDO activity was measured in post-mortem bovine eyes using Yamazaki's method with L-tryptophan as substrate. Because of the physiological importance of IDO in the protection against free oxygen radical damage, a search was conducted to find this enzyme in the eye. Products of tryptophan degradation by IDO, the kynurenine and 3-hydroxyanthranilic acid were detected and measured in the aqueous humor, iris/ciliary body, and the retina by high-performance liquid chromatography (HPLC) coupled with electrochemical detection. IDO activity was 3.2, 9.0 and 10 nmol/mg protein per h for the aqueous humor, iris/ciliary body, and retina, respectively. These findings suggest that, because of its scavenger properties, IDO is involved in the protection of the eye where, because of its transparency, free radicals are formed not only in the normal oxidation process, but also photochemically.
H Z Malina; X D Martin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie     Volume:  231     ISSN:  0721-832X     ISO Abbreviation:  Graefes Arch. Clin. Exp. Ophthalmol.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-11-29     Completed Date:  1993-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  482-6     Citation Subset:  IM    
Universitäts-Augenklinik, CH-8091 Zürich, Switzerland.
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MeSH Terms
3-Hydroxyanthranilic Acid / analysis
Aqueous Humor / enzymology*
Chromatography, High Pressure Liquid
Ciliary Body / enzymology
Indoleamine-Pyrrole 2,3,-Dioxygenase
Iris / enzymology
Kynurenine / analysis
Retina / enzymology*
Tryptophan Oxygenase / metabolism*
Uvea / enzymology*
Reg. No./Substance:
343-65-7/Kynurenine; 548-93-6/3-Hydroxyanthranilic Acid; EC Oxygenase; EC 2,3,-Dioxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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