Document Detail

Indole-3-carbinol enhances anti-proliferative, but not anti-invasive effects of oxaliplatin in colorectal cancer cell lines.
MedLine Citation:
PMID:  18358454     Owner:  NLM     Status:  MEDLINE    
The primary aim of this study was to determine whether combination of the chemopreventive agent indole-3-carbinol (I3C) with oxaliplatin would decrease proliferative index and invasive potential of human colorectal tumour cells. Combination of the agents resulted in a 170-fold decrease in proliferative capacity in SW480 and SW620 cell lines, which was approximately 6-fold greater than for oxaliplatin alone. Decreased proliferation was attributed to enhanced S-phase cell cycle arrest for SW480, and increased apoptosis for SW620 cells. The combined agents resulted in significantly increased E-cadherin levels in SW480 cells, and beta-catenin levels in both cell lines (assessed by in-cell westerns). In SW480 cells confocal microscopy revealed an increase in membrane-associated beta-catenin levels, with oxaliplatin treatments enhancing nuclear export and cytoplasmic localisation. In SW620 cells, all treatments increased membrane localisation of E-cadherin. Whilst both oxaliplatin and I3C decreased invasive capacity of SW480 cells, this was not further enhanced by the combined treatment.
Lynne M Howells; Christopher P Neal; Mhairi C Brown; David P Berry; Margaret M Manson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-17
Journal Detail:
Title:  Biochemical pharmacology     Volume:  75     ISSN:  1873-2968     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-18     Completed Date:  2008-05-27     Revised Date:  2009-05-21    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1774-82     Citation Subset:  IM    
Cancer Biomarkers and Prevention Group, Biocentre, University of Leicester, Leicester LE1 7RH, UK.
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MeSH Terms
Antineoplastic Agents / administration & dosage,  pharmacology*
Apoptosis / drug effects
Cadherins / biosynthesis
Cell Cycle / drug effects
Cell Line, Tumor
Cell Membrane / drug effects,  metabolism
Cell Movement / drug effects
Cell Proliferation / drug effects*
Drug Synergism
Indoles / administration & dosage,  pharmacology*
Organoplatinum Compounds / administration & dosage,  pharmacology*
beta Catenin / biosynthesis
Grant Support
G0100872//Medical Research Council
Reg. No./Substance:
0/Antineoplastic Agents; 0/CTNNB1 protein, human; 0/Cadherins; 0/Indoles; 0/Organoplatinum Compounds; 0/beta Catenin; 63121-00-6/oxaliplatin; 700-06-1/indole-3-carbinol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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