| Indole-3-Acetic Acid Biosynthesis in Fusarium delphinoides Strain GPK, a Causal Agent of Wilt in Chickpea. | |
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MedLine Citation:
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PMID: 23306880 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysis of FDG culture filtrates showed the presence of metabolic intermediates of indole-3-pyruvic acid (IPyA), indole-3-acetamide (IAM), and tryptamine (TRA) pathways. The different IAA biosynthetic pathways were further confirmed by identifying the presence of different enzymes of these pathways. Substrate specificity study of aromatic amino acid aminotransferase revealed that the enzyme is highly specific for tryptophan (Trp) and α-ketoglutarate (α-kg) as amino group donor and acceptor, respectively. Furthermore, the concentration-dependent effect of exogenous IAA on fungal growth was established. Low concentration of exogenous IAA increases the fungal growth and at high concentration it decreases the growth of FDG. |
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Authors:
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Guruprasad B Kulkarni; S Sanjeevkumar; B Kirankumar; M Santoshkumar; T B Karegoudar |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-11 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: - ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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