| Individual variation of human plantar stratum corneum lipids, determined by automated multiple development of high-performance thin-layer chromatography plates. | |
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MedLine Citation:
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PMID: 9174268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The stratum corneum lipids are unique in composition and have been used frequently as a model system of the skin's lipid barrier. Automated multiple development (AMD) of high-performance thin-layer chromatography plates in combination with a 25-step gradient, based on methanol, diethyl ether and n-hexane separated the six major human plantar stratum corneum lipids. Post-chromatographic staining of these lipids with a solution of MnCl2-H2SO4 at 130 degrees C or a solution of CuSO4-H3PO4 at 140 degrees C allowed visualization of the lipids and quantification. The MnCl2-H2SO4 solution stained saturated fatty acids less intensely. Therefore, the CuSO4-H3PO4 solution was used for quantification and we found, on average, 2.06% (w/w) cholesterol 3-sulphate, 20.16% (w/w) free fatty acids, 20.25% (w/w) ceramides, 43.53% (w/w) non-esterified sterols, 4.56% (w/w) triacylglycerols and 9.4% (w/w) sterolesters in the human plantar stratum corneum extracts. The concentration of phospholipids was less than 1% (w/w). In addition, the lipid composition of twenty different human plantar stratum corneum extracts was determined. Statistics revealed a correlation between the ratio of free fatty acids and non-esterified sterols (r=0.832, p<0.01, n=20). Several control experiments proved that this correlation is not due to the extraction method, the post-chromatographic staining procedure or bacterial contamination of the stratum corneum. |
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Authors:
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S Zellmer; J Lasch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 691 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-07-31 Completed Date: 1997-07-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 321-9 Citation Subset: IM |
Affiliation:
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Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chlorides Chromatography, Thin Layer / instrumentation Coloring Agents Copper Sulfate Fatty Acids, Nonesterified / analysis Foot Humans Lipids / analysis*, isolation & purification Manganese Compounds Phosphoric Acids Reproducibility of Results Skin / chemistry* Sterols / analysis Sulfuric Acids |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Coloring Agents; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Manganese Compounds; 0/Phosphoric Acids; 0/Sterols; 0/Sulfuric Acids; 7664-38-2/phosphoric acid; 7664-93-9/sulfuric acid; 7758-98-7/Copper Sulfate; 7773-01-5/manganese chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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