| Indium Tin Oxide devices for amperometric detection of vesicular release by single cells. | |
| | |
MedLine Citation:
|
PMID: 22257976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The microfabrication and successful testing of a series of three ITO (Indium Tin Oxide) microsystems for amperometric detection of cells exocytosis are reported. These microdevices have been optimized in order to simultaneously (i) enhance signal-to-noise ratios, as required electrochemical monitoring, by defining appropriate electrodes geometry and size, and (ii) provide surface conditions which allow cells to be cultured over during one or two days, through apposite deposition of a collagen film. The intrinsic electrochemical quality of the microdevices as well as the effect of different collagen treatments were assessed by investigating the voltammetric responses of two classical redox systems, Ru(NH(3))(6)(3+/2+) and Fe(CN)(6)(3-/4-). This established that a moderate collagen treatment does not incur any significant alteration of voltammetric responses or degradation of the excellent signal-to-noise ratio. Among these three microdevices, the most versatile one involved a configuration in which the ITO microelectrodes were delimited by a microchannel coiled into a spiral. Though providing extremely good electrochemical responses this specific design allowed proper seeding and culture of cells permitting either single cell or cell cluster stimulation and analysis. |
| | |
Authors:
|
Anne Meunier; Rémy Fulcrand; François Darchen; Manon Guille Collignon; Frédéric Lemaître; Christian Amatore |
Related Documents
:
|
21837406 - The dark side of brdu in neural stem cell biology: detrimental effects on cell cycle, d... 21656536 - Cell line misidentification: the case of chang liver cell line. 18509076 - Tonically active inhibition selectively controls feedforward circuits in mouse barrel c... 21757396 - Isolation and characterization of cancer stem-like side population cells in human oral ... 16477646 - Immunohistochemical study of the distribution of sodium-dependent vitamin c transporter... 11299276 - Osteoblasts respond to pulsatile fluid flow with short-term increases in pge(2) but no ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-12-24 |
Journal Detail:
|
Title: Biophysical chemistry Volume: 162 ISSN: 1873-4200 ISO Abbreviation: Biophys. Chem. Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-02-20 Completed Date: 2012-06-12 Revised Date: 2013-02-22 |
Medline Journal Info:
|
Nlm Unique ID: 0403171 Medline TA: Biophys Chem Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 14-21 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
|
Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC « PASTEUR », Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Collagen / chemistry Electrochemical Techniques / instrumentation* Equipment Design Exocytosis* Humans Microelectrodes Microtechnology Signal-To-Noise Ratio Single-Cell Analysis / instrumentation* Surface Properties Tin Compounds / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Tin Compounds; 71243-84-0/indium tin oxide; 9007-34-5/Collagen |
| Comments/Corrections | |
Erratum In:
|
Biophys Chem. 2013 Jan;171:84-5 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sleep propensity at daytime as assessed by Multiple Sleep Latency Tests (MSLT) in patients with schi...
Next Document: Changes in baroreflex control of renal sympathetic nerve activity in high-fat-fed rats as a predicto...