Document Detail


Indirect ultraviolet detection of biologically relevant organic acids by capillary electrophoresis.
MedLine Citation:
PMID:  8998569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Indirect UV detection of fourteen short-chain organic acids (e.g., oxalic acid, citric acid, malonic acid, tartaric acid, methylmalonic acid, alpha-ketoglutaric acid, succinic acid, ethylmalonic acid, methylsuccinic acid, glutaric acid, apidic acid, methylglutaric acid, lactic acid and pyruvic acid) by capillary electrophoresis is described. The method used phthalate as the UV-absorbing additive in carbonate buffer and the non-absorbing analytes were detected indirectly at 230 nm. The influences of buffer pH, ionic strength, concentration of phthalate and organic modifier on indirect signal response and migration behavior of the organic acids were investigated. Comparisons of reproducibility on migration time, limit of detection and separation efficiency among three types of capillary (e.g., polyacrylamide-coated, myristyltrimethylammonium bromide-coated and uncoated capillaries) were conducted. The method developed was applied to detect succinic acid, methylmalonic acid, citric acid, glutaric acid and lactic acid in human body fluids, and preliminary results were provided.
Authors:
H Chen; Y Xu; F Van Lente; M P Ip
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  679     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-01-21     Completed Date:  1997-01-21     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  49-59     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Cleveland State University, OH 44115, USA.
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MeSH Terms
Descriptor/Qualifier:
Buffers
Carbonates / chemistry
Citrates / blood*,  urine
Dicarboxylic Acids / blood*,  urine
Electrophoresis, Capillary / methods*
Humans
Hydrogen-Ion Concentration
Hydroxy Acids / blood*,  urine
Phthalic Acids / chemistry
Reproducibility of Results
Spectrophotometry, Ultraviolet / methods*
Viscosity
Chemical
Reg. No./Substance:
0/Buffers; 0/Carbonates; 0/Citrates; 0/Dicarboxylic Acids; 0/Hydroxy Acids; 0/Phthalic Acids; 497-19-8/sodium carbonate; 88-99-3/phthalic acid

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