Document Detail


Indirect fluorescence determination of lactate and pyruvate in single erythrocytes by capillary electrophoresis.
MedLine Citation:
PMID:  8136909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A scheme of using fluorescein as the fluorophore for indirect detection of anions was demonstrated. This system is quite stable at a fluorescein concentration of 100 microM even without any other buffer components. Different injection modes affect the limit of detection (LOD). A LOD of about 20 amol was obtained for lactate under optimal conditions. Lactate and pyruvate in the intracellular fluid of erythrocytes were measured in this manner. The average amounts in a single erythrocyte for lactate and pyruvate are 1.3 and 2.1 fmol, respectively, or a ratio of 1.6 for pyruvate to lactate. Variations of the absolute amounts and the ratios are fairly large among a group of 27 cells examined. This is consistent with the difference of cells in size and composition. Although the migration times changed by up to 20% during a series of runs from the influence of concomitants in the cells, the migration time ratio was maintained around 1.072 with 3% relative standard deviation.
Authors:
Q Xue; E S Yeung
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  661     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-04-28     Completed Date:  1994-04-28     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  287-95     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Iowa State University, Ames 50011.
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MeSH Terms
Descriptor/Qualifier:
Adult
Electrophoresis / methods*
Erythrocytes / chemistry*
Evaluation Studies as Topic
Fluorescein
Fluoresceins
Humans
Lactates / analysis*
Lactic Acid
Male
Pyruvates / analysis*
Pyruvic Acid
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 2321-07-5/Fluorescein; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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