| Indirect examination of exocrine pancreatic function in vivo by determination of chymotrypsin activity in animal intestine using a synthetic substrate. | |
MedLine Citation:
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PMID: 8704047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using the substrate N-acetyl-L-tyrosyl-p-aminobenzoic acid, we determined chymotrypsin activity in the small intestine of calf, pig, and poultry. Orally administered N-acetyl-L-tyrosyl-p-aminobenzoic acid is enzymatically cleaved in vivo, and the released p-aminobenzoic acid is determined by HPLC. We found that the p-aminobenzoic acid concentration in plasma and urine was significantly influenced by the feeding of soya flour. After soybean flour feeding, the p-aminobenzoic acid concentration significantly increased in the plasma of calves and hens, in contrast to pigs, where the p-aminobenzoic acid concentration significantly decreased. This shows that the oral administration of N-acetyl-L-tyrosyl-p-aminobenzoic acid with subsequent determination of p-aminobenzoic acid is suitable for the estimation of exocrine pancreatic function and for determination of changes in intestinal proteolytic activity caused by antinutritive substances. |
Authors:
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R Kastel; I Rosival; M Bartik; J Blahovec |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies Volume: 34 ISSN: 0939-4974 ISO Abbreviation: Eur J Clin Chem Clin Biochem Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-09-09 Completed Date: 1996-09-09 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9105775 Medline TA: Eur J Clin Chem Clin Biochem Country: GERMANY |
Other Details:
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Languages: eng Pagination: 311-4 Citation Subset: IM |
Affiliation:
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Department of Nutrition, Dietetics and Forage, University of Veterinary Medicine, Kosice, Slovak Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4-Aminobenzoic Acid
/
analogs & derivatives*,
analysis*,
blood,
metabolism,
urine Animal Feed Animals Cattle Chickens Chromatography, High Pressure Liquid Chymotrypsin / metabolism* Feces / chemistry Female Intestines / enzymology* Male Pancreas / enzymology* Swine |
| Chemical | |
Reg. No./Substance:
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150-13-0/4-Aminobenzoic Acid; 38219-60-2/4-(N-acetyltyrosyl)aminobenzoic acid; EC 3.4.21.1/Chymotrypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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