Document Detail

Indirect examination of exocrine pancreatic function in vivo by determination of chymotrypsin activity in animal intestine using a synthetic substrate.
MedLine Citation:
PMID:  8704047     Owner:  NLM     Status:  MEDLINE    
Using the substrate N-acetyl-L-tyrosyl-p-aminobenzoic acid, we determined chymotrypsin activity in the small intestine of calf, pig, and poultry. Orally administered N-acetyl-L-tyrosyl-p-aminobenzoic acid is enzymatically cleaved in vivo, and the released p-aminobenzoic acid is determined by HPLC. We found that the p-aminobenzoic acid concentration in plasma and urine was significantly influenced by the feeding of soya flour. After soybean flour feeding, the p-aminobenzoic acid concentration significantly increased in the plasma of calves and hens, in contrast to pigs, where the p-aminobenzoic acid concentration significantly decreased. This shows that the oral administration of N-acetyl-L-tyrosyl-p-aminobenzoic acid with subsequent determination of p-aminobenzoic acid is suitable for the estimation of exocrine pancreatic function and for determination of changes in intestinal proteolytic activity caused by antinutritive substances.
R Kastel; I Rosival; M Bartik; J Blahovec
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies     Volume:  34     ISSN:  0939-4974     ISO Abbreviation:  Eur J Clin Chem Clin Biochem     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-09-09     Completed Date:  1996-09-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9105775     Medline TA:  Eur J Clin Chem Clin Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  311-4     Citation Subset:  IM    
Department of Nutrition, Dietetics and Forage, University of Veterinary Medicine, Kosice, Slovak Republic.
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MeSH Terms
4-Aminobenzoic Acid / analogs & derivatives*,  analysis*,  blood,  metabolism,  urine
Animal Feed
Chromatography, High Pressure Liquid
Chymotrypsin / metabolism*
Feces / chemistry
Intestines / enzymology*
Pancreas / enzymology*
Reg. No./Substance:
150-13-0/4-Aminobenzoic Acid; 38219-60-2/4-(N-acetyltyrosyl)aminobenzoic acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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