| Indirect electrocatalytic determination of choline by monitoring hydrogen peroxide at the choline oxidase-prussian blue modified iron phosphate nanostructures. | |
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MedLine Citation:
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PMID: 22119562 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Choline, as a marker of cholinergic activity in brain tissue, is very important in biological and clinical analysis, especially in the clinical detection of the neurodegenerative disorders disease. This work presents an electrochemical approach for the detection of choline based on prussian blue modified iron phosphate nanostructures (PB-FePO(4)). The obtained nanostructures showed a good catalysis toward the electroreduction of H(2)O(2), and an amperometric choline biosensor was developed by immobilizing choline oxidase on the PB-FePO(4) nanostructures. The biosensor exhibited a rapid response (ca. 2s), low detection limit (0.4±0.05μM), wide linear range (2μM to 3.2mM), high sensitivity (∼75.2μAmM(-1)cm(-2)), as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, uric acid and 4-acetamidophenol did not cause obvious interference due to the low detection potential (-0.05V versus saturated calomel electrode). This nanostructure could be used as a promise platform for the construction of other oxidase-based biosensors. |
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Authors:
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Hui Zhang; Yajing Yin; Ping Wu; Chenxin Cai |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-9 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: - ISSN: 1873-4235 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Jiangsu Key Laboratory of New Power Batteries, Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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