Document Detail

Independent regulation by sodium butyrate of gonadotropin alpha gene expression and cell cycle progression in HeLa cells.
MedLine Citation:
PMID:  6203027     Owner:  NLM     Status:  MEDLINE    
Sodium butyrate alters the growth and gene expression of a variety of differentiating and neoplastic cell types. For example, addition of 5 mM butyrate to HeLa cells is reported to both induce gonadotropin alpha subunit biosynthesis and block cell cycling in G1. We have studied these two actions of butyrate on HeLa cells and found that they are regulated in distinct ways. The induction of alpha subunit synthesis was due to an increase in the rate of transcription of the alpha gene. Using synchronized populations of HeLa cells, we determined that butyrate stimulates alpha transcription throughout the cell cycle. In contrast, treated cells arrest in G1 only if exposed to butyrate for a discrete period during the previous S phase. We conclude that butyrate inhibits DNA synthesis through a cell cycle-specific action that is independent from its direct action to stimulate transcription of the gonadotropin alpha gene.
R B Darnell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  4     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-07-09     Completed Date:  1984-07-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  829-39     Citation Subset:  IM    
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MeSH Terms
Butyrates / pharmacology*
Butyric Acid
Butyric Acids / pharmacology*
Cell Cycle / drug effects*
Chorionic Gonadotropin / genetics*
Genes / drug effects*
Glycoprotein Hormones, alpha Subunit
Hela Cells / drug effects,  physiology
Histones / genetics
Interphase / drug effects
Peptide Fragments / genetics*
RNA, Messenger / genetics
Transcription, Genetic / drug effects
Grant Support
Reg. No./Substance:
0/Butyrates; 0/Butyric Acids; 0/Chorionic Gonadotropin; 0/Glycoprotein Hormones, alpha Subunit; 0/Histones; 0/Peptide Fragments; 0/RNA, Messenger; 107-92-6/Butyric Acid

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