| Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes. | |
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MedLine Citation:
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PMID: 20135119 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biodiesel is an interesting alternative energy source and is used as substitute for petroleum-based diesel. Microorganisms have been used for biodiesel production due to their significant environmental and economic benefits. However, few researches have investigated the regulation of fatty acid composition of these microbial diesels. Fatty acid biosynthesis in Escherichia coli has provided a paradigm for other bacteria and plants. By overexpressing two genes (fabA and fabB) associated with unsaturated fatty acid (UFA) synthesis in E. coli, we have engineered an efficient producer of UFAs. Saturated fatty acid (SFA) contents decreased from 50.2% (the control strain) to 34.6% (the recombinant strain overexpressing fabA and fabB simultaneously) and the ratio of cis-vaccenate (18:1Delta11), a major UFA in E. coli, reached 51.1% in this recombinant strain. When an Arabidopsis thaliana thioesterase (AtFatA) was coexpressed with these two genes, 0.19 mmol l(-1) fatty acids was produced by this E. coli strain after 18-h culture under shake-flask conditions. Free fatty acids made up about 37.5% of total fatty acid concentration in this final engineered strain carrying fabA, fabB, and AtFatA, and the ratio of UFA/SFA reached 2.3:1. This approach offers a means to improve the fatty acid composition of microdiesel and might pave the way for production of biodiesel equivalents using engineered microorganisms in the near future. |
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Authors:
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Yujin Cao; Jianming Yang; Mo Xian; Xin Xu; Wei Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-05 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 87 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-18 Completed Date: 2010-10-04 Revised Date: 2011-04-05 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 271-80 Citation Subset: IM |
Affiliation:
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Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, 266101 Qingdao, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
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genetics*,
metabolism Arabidopsis Proteins / genetics*, metabolism Escherichia coli / genetics*, metabolism Escherichia coli Proteins / genetics*, metabolism Fatty Acids / biosynthesis Fatty Acids, Unsaturated / biosynthesis* Gene Expression Genetic Engineering* Hydro-Lyases / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Escherichia coli Proteins; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; EC 2.3.1.41/3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; EC 2.3.1.41/FabB protein, E coli; EC 4.2.1.-/Hydro-Lyases; EC 4.2.1.60/3-hydroxydecanoyl-(acyl-carrier-protein) dehydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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