| Increasing the sensitivity of Listeria monocytogenes assays: evaluation using ELISA and amperometric detection. | |
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MedLine Citation:
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PMID: 10605890 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An immunosensor for the detection of Listeria monocytogenes was developed. ELISA and amperometric studies were run in parallel to develop a more sensitive and rapid assay for the bacterium. Conditions for the immunosensor were primarily characterised using ELISA. A direct sandwich assay was employed and the affinities of two polyclonal (goat and rabbit) and one monoclonal (mouse) anti-L. monocytogenes antibodies were compared using this format. Owing to low sensitivity being obtained with all antibodies, biotin-avidin amplification and an indirect sandwich assay were employed. The system was then transferred to screen-printed electrodes (SPEs), the primary antibody being immobilised by cross-linking with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, and the mode of detection being amperometric. Various parameters (limit of detection, working range, incubation time, cross-reactivity) of the systems were characterised. The effect of direct incubation in milk is also discussed. The final immunosensor had a working range of 1 x 10(6)-1 x 10(3) cells ml-1 and a detection limit of 9 x 10(2) cells ml-1. The assay took about 3.5 h to complete. |
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Authors:
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E L Crowley; C K O'Sullivan; G G Guilbault |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Analyst Volume: 124 ISSN: 0003-2654 ISO Abbreviation: Analyst Publication Date: 1999 Mar |
Date Detail:
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Created Date: 2000-01-11 Completed Date: 2000-01-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 295-9 Citation Subset: IM |
Affiliation:
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Laboratory of Sensor Development, National University of Ireland Cork, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques Enzyme-Linked Immunosorbent Assay Humans Listeria monocytogenes / isolation & purification* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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