| Increasing the "region of interest" and "time of interest", both reduce the variability of blood flow measurements using laser speckle contrast imaging. | |
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MedLine Citation:
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PMID: 21439303 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Objective Objective: Both spatial variability and temporal variability of skin blood flow are high. Laser Speckle contrast imagers (LSCI) allow non-contact, real-time recording of cutaneous blood flow on large skin surfaces. Thereafter, the observer can define different sizes for the region of interest (ROI) in the images to decrease spatial variability and different durations over which the blood flow values are averaged (time of interest, TOI) to decrease temporal variability. We aimed to evaluate the impact of the choices of ROI and TOI on the analysis of rest blood flow and post occlusive reactive hyperemia (PORH). METHODS: Cutaneous blood flow (CBF) was assessed at rest and during PORH. Three different sizes of ROI (1 mm(2), 10 mm(2) and 100 mm(2)), and three different TOI (CBF averaged over 1s, 15s, and 30s for rest, and over 1s, 5s and 10s for PORH peak) were evaluated. Inter-subjects and intra-subjects coefficient of variations (inter-CV and intra-CV) were studied. RESULTS: The inter-subject variability of CBF is about 25% at rest and is moderately improved when the size of the ROI increases (inter-CV=31%, for 1s and 1 mm(2) vs inter-CV=23%, for 15s and 100 mm(2)). However, increasing the TOI does not improve the results. The variability of the PORH peak is lower with an inter-CV varying between 11.4% (10s and 100 mm(2)) and 21.6% (5s and 1 mm(2)). The lowest intra-CV for the CBF at rest was 7.3% (TOI of 15s on a ROI of 100 mm(2)) and was 3.1% for the PORH peak (TOI of 10s on a ROI of 100 mm(2)). CONCLUSION: We suggest that a size of ROI larger than10 mm(2) and a TOI longer than 1s are required to reduce the variability of CBF measurements both at rest and during PORH peak evaluations at the forearm level. Many technical aspects such as comparison of laser speckle contrast imaging and laser Doppler imaging or the effect of skin to head distance on recorded values with LCSI are required to improve future studies using this fascinating clinical tool. |
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Authors:
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Pascal Rousseau; Guillaume Mahé; Firas Haj Yassin; Sylvain Durand; Anne Humeau; Georges Leftheriotis; Pierre Abraham |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-22 |
Journal Detail:
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Title: Microvascular research Volume: - ISSN: 1095-9319 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2010. Published by Elsevier Inc. |
Affiliation:
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Laboratory of Vascular Investigations, University Hospital of Angers, France; Department of Plastic Surgery, University Hospital of Angers, France; Biologie Neurovasculaire Intégrée (BNVI) - Unité mixte UMR CNRS 6214 / INSERM 771, Faculté de Médecine, Angers, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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