Document Detail


Increasing protein at the expense of carbohydrate in the diet down-regulates glucose utilization as glucose sparing effect in rats.
MedLine Citation:
PMID:  21326875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High protein (HP) diet could serve as a good strategy against obesity, provoking the changes in energy metabolic pathways. However, those modifications differ during a dietary adaptation. To better understand the mechanisms involved in effect of high protein diet (HP) on limiting adiposity in rats we studied in parallel the gene expression of enzymes involved in protein and energy metabolism and the profiles of nutrients oxidation. Eighty male Wistar rats were fed a normal protein diet (NP, 14% of protein) for one week, then either maintained on NP diet or assigned to a HP diet (50% of protein) for 1, 3, 6 and 14 days. mRNA levels of genes involved in carbohydrate and lipid metabolism were measured in liver, adipose tissues, kidney and muscles by real time PCR. Energy expenditure (EE) and substrate oxidation were measured by indirect calorimetry. Liver glycogen and plasma glucose and hormones were assayed. In liver, HP feeding 1) decreased mRNA encoding glycolysis enzymes (GK, L-PK) and lipogenesis enzymes(ACC, FAS), 2) increased mRNA encoding gluconeogenesis enzymes (PEPCK), 3) first lowered, then restored mRNA encoding glycogen synthesis enzyme (GS), 4) did not change mRNA encoding β-oxidation enzymes (CPT1, ACOX1, βHAD). Few changes were seen in other organs. In parallel, indirect calorimetry confirmed that following HP feeding, glucose oxidation was reduced and fat oxidation was stable, except during the 1(st) day of adaptation where lipid oxidation was increased. Finally, this study showed that plasma insulin was lowered and hepatic glucose uptake was decreased. Taken together, these results demonstrate that following HP feeding, CHO utilization was increased above the increase in carbohydrate intake while lipogenesis was decreased thus giving a potential explanation for the fat lowering effect of HP diets.
Authors:
Magdalena Stepien; Claire Gaudichon; Gilles Fromentin; Patrick Even; Daniel Tomé; Dalila Azzout-Marniche
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-07
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-02-17     Completed Date:  2011-09-01     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e14664     Citation Subset:  IM    
Affiliation:
INRA/AgroParisTech, CNRH-IdF, UMR914 Nutrition Physiology and Ingestive Behavior, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Carbohydrate Metabolism / drug effects*,  physiology
Diet, Carbohydrate-Restricted / adverse effects
Dietary Carbohydrates / pharmacology*
Dietary Proteins / pharmacology*
Glucose / metabolism*
Lipid Metabolism / drug effects
Liver / drug effects,  metabolism
Male
Models, Biological
Oxidation-Reduction / drug effects
Rats
Rats, Wistar
Up-Regulation
Chemical
Reg. No./Substance:
0/Dietary Carbohydrates; 0/Dietary Proteins; 50-99-7/Glucose
Comments/Corrections
Erratum In:
PLoS One. 2011;6(3). doi: 10.1371/annotation/ad8aa7d5-17c1-483d-8b69-610c8839bc3a

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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