Document Detail


Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry.
MedLine Citation:
PMID:  22766096     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Confocal immunofluorescence tiling imaging revealed the spatio-temporal distributions of osterix and sclerostin in femurs from3-day-old, 2-week-old and 4-week-old rats to be reciprocally exclusive at the tissue level. Further quantitative three-dimensional immuno fluorescence morphometry demonstrated the increasing distribution of sclerostin in the osteocytic lacuno-canalicular system specifically in diaphysis, which paralleled the cooperative participation and depletion of osterix and β-catenin in adjacent periosteum cells. Treating MC3T3-E1 cells with BIO (a GSK3 inhibitor) induced the stabilization of β-catenin and nuclear translocation of osterix, and negatively regulated osteocalcin/BGLAP and Dmp1. These results collectively demonstrate that the increasing distribution of sclerostin in diaphyseal cortical bone appears to be involved in the attenuation of osterix and β-catenin in adjacent periosteum cells, thus possibly contributing to osteoblast maturation and reducing the osteoblast formation at this bone site. Our confocal microscopy-based imaging analyses provide a comprehensive and detailed view of the spatio-temporal distribution of sclerostin, β-catenin and osterix at the tissue to subcellular level in a coherent manner, and uncovered their spatio-temporal cooperation in postnatal bone development, thus providing evidence that they link skeletogenic growth and functional bone development.
Authors:
Takashi Watanabe; Yoshihiro Tamamura; Akiyoshi Hoshino; Yuji Makino; Hiroshi Kamioka; Teruo Amagasa; Akira Yamaguchi; Tadahiro Iimura
Related Documents :
25457786 - Iliac bone grafting of the intact glenoid improves shoulder stability with optimal graf...
9418616 - The ranawat award. histology of nine structural bone grafts used in total knee arthropl...
22793956 - Histomorphometric analysis of maxillary sinus augmentation using an alloplast bone subs...
8863026 - Quantitative analysis of cancellous bone graft available from the greater trochanter.
24857486 - Creep of trabecular bone from the human proximal tibia.
24095596 - Total elbow arthroplasty for acute distal humeral fractures in patients over 65 years o...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-2
Journal Detail:
Title:  Bone     Volume:  -     ISSN:  1873-2763     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8504048     Medline TA:  Bone     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Section of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University Japan; Global Center of Excellence (GCOE) program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University Japan; Section of Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Japan; Department of Molecular and Cellular Biochemistry, Osaka University, Graduate School of Dentistry, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  MEPE is a novel regulator of growth plate cartilage mineralization.
Next Document:  Innate immune responses to rhinovirus are reduced by the high-affinity IgE receptor in allergic asth...