| Increasing p53 protein sensitizes non-small cell lung cancer to paclitaxel and cisplatin in vitro. | |
| | |
MedLine Citation:
|
PMID: 20944137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIM: To determine whether increasing p53 protein levels confers enhanced chemosensitivity in non-small cell lung cancer (NSCLC). MATERIALS AND METHODS: Three NSCLC cell lines, with different endogenous p53 expression, were transfected with wild-type p53 (wt-p53) or CD-1 (truncated wt-p53) genes. Cells were subsequently treated with cisplatin (CDDP) or paclitaxel (PAX). Cell viability was measured using Alamar Blue Assay. RESULTS: Cells transfected with CD-1 expressed 13-38% higher levels of p53 protein compared to cells transfected with the wt-p53 gene, despite their baseline endogenous levels. CD-1-transfected cells also had higher cell death when treated with CDDP (p<0.05) or PAX, exhibiting 30-60% higher death rates than cells transfected with the wt-p53 gene and 130-160% higher than untransfected cells. A significant positive correlation between p53 protein concentration and cytotoxic response was demonstrated (R(2) for CDDP=0.823; R(2) for PAX=0.909; p<0.001). CONCLUSION: Increasing intracellular p53 protein concentrations can augment the effect of CDDP and PAX in NSCLC, despite the baseline level of p53 protein expression. |
| | |
Authors:
|
Vamsi P Guntur; J Clifford Waldrep; Jennifer J Guo; Kim Selting; Rajiv Dhand |
Related Documents
:
|
15529177 - Promyelocytic leukemia protein-induced growth suppression and cell death in liver cance... 14693837 - Analysis of cell cycle regulator proteins in non-small cell lung cancer. 9659517 - Isolation and characterisation of radioresistant human kb carcinoma cells in vitro. 7915517 - Transcriptional regulation of the pcna promoter by p53. 18634577 - No pun intended: future directions in invertebrate glial cell migration studies. 17093407 - A protein interaction network for pluripotency of embryonic stem cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Anticancer research Volume: 30 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-10-14 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
|
Languages: eng Pagination: 3557-64 Citation Subset: IM |
Affiliation:
|
Division of Pulmonary, Critical Care, and Environmental Medicine, School of Medicine, University of Missouri, Five Hospital Drive, Columbia, MO 65212, USA. gunturv@health.missouri.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antineoplastic Agents
/
pharmacology* Blotting, Western Carcinoma, Non-Small-Cell Lung / genetics, metabolism* Cell Line, Tumor Cell Survival / drug effects Cisplatin / pharmacology Drug Resistance, Neoplasm / genetics* Enzyme-Linked Immunosorbent Assay Humans Lung Neoplasms / genetics, metabolism* Paclitaxel / pharmacology Transfection Tumor Suppressor Protein p53 / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents; 0/Tumor Suppressor Protein p53; 15663-27-1/Cisplatin; 33069-62-4/Paclitaxel |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Houttuynia cordata Thunb Extract Inhibits Cell Growth and Induces Apoptosis in Human Primary Colorec...
Next Document: Inhibition of Heat-shock Protein 27 Expression Eliminates Drug Resistance of Osteosarcoma to Zoledro...