| Increasing mitochondrial superoxide dismutase abundance leads to impairments in protein quality control and ROS scavenging systems and to lifespan shortening. | |
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MedLine Citation:
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PMID: 20080171 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fungal aging model Podospora anserina contains three superoxide dismutases (SODs) in different cellular compartments. While PaSOD1 represents the Cu/Zn isoform located in the cytoplasm and in the mitochondrial inter-membrane space, PaSOD2 localizes to the perinuclear ER. PaSOD3, a protein with a manganese binding domain and a mitochondrial targeting sequence (MTS) is the mitochondrial SOD. Over-expression of PaSod3 leads to lifespan reduction and increased sensitivity against paraquat and hydrogen peroxide. The negative effects of PaSod3 over-expression correlate with a strong reduction in the abundance of mitochondrial peroxiredoxin, PaPRX1, and the matrix protease PaCLPP disclosing impairments of mitochondrial quality control and ROS scavenging pathways in PaSod3 over-expressors. Deletion of PaSod3 leads to increased paraquat sensitivity while hydrogen peroxide sensitivity and lifespan are not significantly changed when compared to the wild-type strain. These latter characteristics are unexpected and challenge the 'mitochondrial free radical theory of aging'. |
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Authors:
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Sandra Zintel; Denise Schwitalla; Karin Luce; Andrea Hamann; Heinz D Osiewacz |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-18 |
Journal Detail:
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Title: Experimental gerontology Volume: 45 ISSN: 1873-6815 ISO Abbreviation: Exp. Gerontol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-06-01 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 525-32 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Institute of Molecular Biosciences and Cluster of Excellence Macromolecular Complexes, Department of Biosciences, J.W. Goethe-University, Max-von-Laue-Strasse 9, Frankfurt am Main, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA, Fungal / genetics Free Radical Scavengers / metabolism Fungal Proteins / genetics, metabolism Gene Expression Genes, Fungal Green Fluorescent Proteins / genetics, metabolism Hydrogen Peroxide / pharmacology Mitochondria / metabolism* Models, Biological Mutation Paraquat / pharmacology Podospora / drug effects, genetics, metabolism* Reactive Oxygen Species / metabolism Recombinant Fusion Proteins / genetics, metabolism Superoxide Dismutase / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal; 0/Free Radical Scavengers; 0/Fungal Proteins; 0/Reactive Oxygen Species; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 4685-14-7/Paraquat; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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