Document Detail

Increasing histone acetylation of cloned embryos, but not donor cells, by sodium butyrate improves their in vitro development in pigs.
MedLine Citation:
PMID:  20132017     Owner:  NLM     Status:  MEDLINE    
Previous studies have demonstrated that increased histone acetylation in donor cells or cloned embryos, by applying a histone deacetylase inhibitor (HDACi) such as trichostatin A (TSA), significantly enhances their developmental competence. However, its effect may vary with the type of HDACi and the target species, with some research showing nonsignificant or detrimental effects of TSA on in vitro and in vivo development of embryos. In this study, we show that sodium salt of butyric acid, a short-chain fatty acid produced naturally in the body by bacterial degradation of dietary fibers in the colon and rectum, increases histone acetylation in pig fibroblast and embryos at a concentration of 1.0 and 5.0 mM, respectively. However, treatment of donor cells with NaBu did not affect the rate of blastocyst formation or embryo quality in terms of histone acetylation and total nuclei per blastocyst (p > 0.05). On the contrary, treatment of cloned pig embryos with NaBu for 4 h significantly enhanced (p < 0.01) the rate of blastocyst formation (18.3 +/- 2.1 vs. 11.2 +/- 3.0%), although the total nuclei number per blastocyst did not differ. More importantly, blastocysts generated from NaBu-treated cloned embryos had increased levels of histone acetylation that was comparable to those of in vitro fertilized (IVF) embryos (36.7 +/- 3.6 vs. 45.9 +/- 2.5). In conclusion, our data suggest that histone hyperacetylation by NaBu treatment of cloned embryos, but not donor cell, enhances their in vitro development up to blastocyst stage.
Ziban Chandra Das; Mukesh Kumar Gupta; Sang Jun Uhm; Hoon Taek Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular reprogramming     Volume:  12     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-05     Completed Date:  2010-05-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  95-104     Citation Subset:  IM    
Department of Bioscience and Biotechnology, Bio-Organ Research Center/Animal Resources Research Center, Konkuk University, Seoul, South Korea.
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MeSH Terms
Acetylation / drug effects
Blastocyst / drug effects
Butyrates / pharmacology*
Cell Nucleus / metabolism
Cloning, Organism*
Dose-Response Relationship, Drug
Embryo Culture Techniques
Embryo, Mammalian / drug effects,  metabolism
Embryonic Development / drug effects*
Fluorescent Dyes / metabolism
Histones / metabolism*
Indoles / metabolism
Swine / metabolism*
Time Factors
Reg. No./Substance:
0/Butyrates; 0/Fluorescent Dyes; 0/Histones; 0/Indoles; 47165-04-8/DAPI

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