Document Detail

Increasing genomic information in bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution.
MedLine Citation:
PMID:  18054177     Owner:  NLM     Status:  MEDLINE    
The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300-3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps.
Arnaud Tanguy; Nicolas Bierne; Carlos Saavedra; Benjamin Pina; Evelyne Bachère; Michael Kube; Eric Bazin; François Bonhomme; Pierre Boudry; Viviane Boulo; Isabelle Boutet; Leonor Cancela; Carole Dossat; Pascal Favrel; Arnaud Huvet; Sergio Jarque; Didier Jollivet; Sven Klages; Sylvie Lapègue; Ricardo Leite; Jeanne Moal; Dario Moraga; Richard Reinhardt; Jean-François Samain; Eleftherios Zouros; Adelino Canario
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-25
Journal Detail:
Title:  Gene     Volume:  408     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-07     Completed Date:  2008-05-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  27-36     Citation Subset:  IM    
UMR CNRS 7144 UPMC Evolution et Génétique des Populations Marines, Station Biologique, Université Européenne de Bretagne, BP 74, Place Georges Teissier, 29682, Roscoff, France.
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MeSH Terms
Bivalvia / genetics*,  physiology
Evolution, Molecular*
Expressed Sequence Tags*
Gene Library
Genetic Markers
Microsatellite Repeats
Polymorphism, Single Nucleotide
Tandem Repeat Sequences
Reg. No./Substance:
0/Genetic Markers

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