| Increases in intracavernous penile pressure following injections of excitatory amino acid receptor agonists in the hypothalamic paraventricular nucleus of anesthetized rats. | |
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MedLine Citation:
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PMID: 11025771 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: We examined whether N-methyl-D-aspartate (NMDA), amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) or trans-1-amino-1,3-cyclo-pentadicarboxylic acid (ACPD) increase intracavernous pressures when injected in the paraventricular nucleus of the hypothalamus. MATERIALS AND METHODS: Sprague-Dawley rats weighing 250 to 300 gm. were anesthetized with pentobarbital and placed in a stereotaxic apparatus after catheterization of the carotid artery and insertion of a 25 gauge needle in the corpus cavernosum. Both catheters were coupled to pressure transducer systems. Electrical stimulations with glass tungsten microelectrodes and chemical injections with a 0.5 microl. syringe were done in the paraventricular nucleus. At the end of each experiment rats were perfused with saline and 10% formalin solutions. Brains slices 16 microm. thick were mounted and stained with cresyl violet to identify chemical and electrical stimulation sites. RESULTS: Electrical stimulations of the paraventricular nucleus increased intracavernous pressure from 15.5 +/- 5.7 to 50.02 +/- 20.4 cm. H2O in 10 rats (p <0.001) and decreased central pressure in 10 (p <0. 001). Intracavernous pressure increased from 17.2 +/- 5.26 to 60.6 +/- 14.36 cm. H2O with NMDA, 26.0 +/- 8.9 to 49.6 +/- 12.2 cm. H2O with AMPA and 18.0 +/- 2.3 to 41.0 +/- 22.68 cm. H2O with ACPD when injected in the paraventricular nucleus of 5 rats (analysis of variance 12.18, df 3, p <0.007). Mean delays and durations varied between 3.6 to 4.0 and 5.6 to 7.0, respectively, and were similar for all agonists (analysis of variance not significant). CONCLUSIONS: Glutamate receptor subtypes NMDA, ACPD and AMPA cause an increase in intracavernous pressure when injected in the paraventricular nucleus. Therefore, increases in intracavernous pressure following injection of glutamate must be initiated by the synergistic or additive effects of all of these receptor subtypes. |
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Authors:
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A R Zahran; P Vachon; F Courtois; S Carrier |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of urology Volume: 164 ISSN: 0022-5347 ISO Abbreviation: J. Urol. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-21 Completed Date: 2000-11-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376374 Medline TA: J Urol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1793-7 Citation Subset: AIM; IM |
Affiliation:
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Lady Davis Institute of the Jewish General Hospital, Department of Medicine, McGill University, Montreal, Quebec, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electric Stimulation Excitatory Amino Acid Antagonists / pharmacology Male N-Methylaspartate / physiology Paraventricular Hypothalamic Nucleus / physiology* Penile Erection / physiology* Rats Rats, Sprague-Dawley Receptors, Glutamate / physiology* Receptors, N-Methyl-D-Aspartate / physiology |
| Chemical | |
Reg. No./Substance:
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0/Excitatory Amino Acid Antagonists; 0/Receptors, Glutamate; 0/Receptors, N-Methyl-D-Aspartate; 6384-92-5/N-Methylaspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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