| Increases in guanylin and uroguanylin in a mouse model of osmotic diarrhea are guanylate cyclase C-independent. | |
| | |
MedLine Citation:
|
PMID: 11677212 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND & AIMS: Guanylin and uroguanylin are peptide hormones that are homologous to the diarrhea-causing Escherichia coli enterotoxins. These secretagogues are released from the intestinal epithelia into the intestinal lumen and systemic circulation and bind to the receptor guanylate cyclase C (GC-C). We hypothesized that a hypertonic diet would result in osmotic diarrhea and cause a compensatory down-regulation of guanylin/uroguanylin. METHODS: Gut-to-carcass weights were used to measure fluid accumulation in the intestine. Northern and/or Western analysis was used to determine the levels of guanylin, uroguanylin, and GC-C in mice with osmotic diarrhea. RESULTS: Wild-type mice fed a polyethylene glycol or lactose-based diet developed weight loss, diarrhea, and an increased gut-to-carcass ratio. Unexpectedly, 2 days on either diet resulted in increased guanylin/uroguanylin RNA and prohormone throughout the intestine, elevated uroguanylin RNA, and prohormone levels in the kidney and increased levels of circulating prouroguanylin. GC-C-deficient mice given the lactose diet reacted with higher gut-to-carcass ratios. Although they did not develop diarrhea, GC-C-sufficient and -deficient mice on the lactose diet responded with elevated levels of guanylin and uroguanylin RNA and protein. A polyethylene glycol drinking water solution resulted in diarrhea, higher gut-to-carcass ratios, and induction of guanylin and uroguanylin in both GC-C heterozygous and null animals. CONCLUSIONS: We conclude that this model of osmotic diarrhea results in a GC-C-independent increase in intestinal fluid accumulation, in levels of these peptide ligands in the epithelia of the intestine, and in prouroguanylin in the kidney and blood. |
| | |
Authors:
|
K A Steinbrecher; E A Mann; R A Giannella; M B Cohen |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Gastroenterology Volume: 121 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2001 Nov |
Date Detail:
|
Created Date: 2001-10-25 Completed Date: 2001-12-05 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
|
Languages: eng Pagination: 1191-202 Citation Subset: AIM; IM |
Affiliation:
|
Division of Pediatric Gastroenterology, Hepatology and Nutrition and Graduate Program in Molecular and Developmental Biology, Children's Hospital Research Foundation, Cincinnati, Ohio, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antiporters* Carrier Proteins / genetics Diarrhea / metabolism* Female Gastrointestinal Hormones* Guanylate Cyclase* Intestines / metabolism Kidney / metabolism Membrane Proteins / genetics Mice Mice, Inbred C57BL Natriuretic Peptides Osmotic Pressure Peptides / analysis*, genetics, physiology RNA, Messenger / analysis Receptors, Cell Surface / physiology* Receptors, Peptide* Sodium-Hydrogen Antiporter / genetics |
| Grant Support | |
ID/Acronym/Agency:
|
DK 47318/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antiporters; 0/Carrier Proteins; 0/Gastrointestinal Hormones; 0/Membrane Proteins; 0/Natriuretic Peptides; 0/Peptides; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, Peptide; 0/Slc26a3 protein, mouse; 0/Sodium-Hydrogen Antiporter; 0/sodium-hydrogen exchanger 3; 140653-38-9/guanylin; 152175-68-3/uroguanylin; EC 4.6.1.2/Guanylate Cyclase; EC 4.6.1.2/enterotoxin receptor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hepatic uptake of cholecystokinin octapeptide by organic anion-transporting polypeptides OATP4 and O...
Next Document: Expression and localization of the multidrug resistance proteins MRP2 and MRP3 in human gallbladder ...