Document Detail

Increases in cell elongation, plastid compartment size and phytoene synthase activity underlie the phenotype of the high pigment-1 mutant of tomato.
MedLine Citation:
PMID:  12844264     Owner:  NLM     Status:  MEDLINE    
A characteristic trait of the high pigment-1 ( hp-1) mutant phenotype of tomato ( Lycopersicon esculentum Mill.) is increased pigmentation resulting in darker green leaves and a deeper red fruit. In order to determine the basis for changes in pigmentation in this mutant, cellular and plastid development was analysed during leaf and fruit development, as well as the expression of carotenogenic genes and phytoene synthase enzyme activity. The hp-1 mutation dramatically increases the periclinal elongation of leaf palisade mesophyll cells, which results in increased leaf thickness. In addition, in both palisade and spongy mesophyll cells, the total plan area of chloroplasts per cell is increased compared to the wild type. These two perturbations in leaf development are the primary cause of the darker green hp-1 leaf. In the hp-1 tomato fruit, the total chromoplast area per cell in the pericarp cells of the ripe fruit is also increased. In addition, although expression of phytoene synthase and desaturase is not changed in hp-1 compared to the wild type, the activity of phytoene synthase in ripe fruit is 1.9-fold higher, indicating translational or post-translational control of carotenoid gene expression. The increased plastid compartment size in leaf and fruit cells of hp-1 is novel and provides evidence that the normally tightly controlled relationship between cell expansion and the replication and expansion of plastids can be perturbed and thus could be targeted by genetic manipulation.
P J Cookson; J W Kiano; C A Shipton; P D Fraser; S Romer; W Schuch; P M Bramley; K A Pyke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-07-03
Journal Detail:
Title:  Planta     Volume:  217     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-01     Completed Date:  2004-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  896-903     Citation Subset:  IM    
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK.
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MeSH Terms
Alkyl and Aryl Transferases / genetics,  metabolism*
Base Sequence
Carotenoids / metabolism
Chlorophyll / metabolism
DNA Primers
Lycopersicon esculentum / genetics*,  growth & development*,  ultrastructure
Oxidoreductases / genetics,  metabolism
Plant Leaves / enzymology
Plastids / ultrastructure*
Polymerase Chain Reaction
Seeds / physiology
Reg. No./Substance:
0/DNA Primers; 1406-65-1/Chlorophyll; 36-88-4/Carotenoids; EC 1.-/Oxidoreductases; EC 1.14.99.-/phytoene dehydrogenase; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.1.-/phytoene synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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