Document Detail


Increased yield of myeloid progenitor cells in bone marrow harvested for autologous transplantation by pretreatment with recombinant human granulocyte-colony stimulating factor.
MedLine Citation:
PMID:  1384899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pretreatment with haemopoietic cytokines prior to marrow harvest may result in improved quality of bone marrow harvested for autologous bone marrow transplantation (BMT). Such improvements may reduce the risk for graft failure and decrease time to engraftment. Patients undergoing autologous BMT received recombinant human G-CSF (rhG-CSF) immediately prior to marrow harvest. rhG-CSF was administered as daily subcutaneous injections for 5 days at 5 micrograms/kg body weight. Comparison of bone marrow samples before and after rhG-CSF treatment showed an increased bone marrow cellularity and a ninefold increase in the number of marrow leucocytes per volume aspirated. The mean marrow myeloid:erythroid ratio increased from 2.6 to 4.0. The mean numbers of immature (CD38 positive) and proliferating (CD71 positive) myeloid cells increased significantly from 41.6 to 50.8% and from 17.0 to 34.8%, respectively. Other subsets studied, including CD34 positive stem cells, were unchanged. The relative numbers of day 7 and 14 granulocyte-macrophage colony-forming units (day 7/14 GM-CFU) were unchanged. Long-term marrow cultures revealed that the numbers of 'long-term culture initiating cells' were unchanged after rhG-CSF treatment in spite of the ninefold increase in cellularity. To date, five of the patients have been transplanted with autologous marrow harvested after rhG-CSF treatment. Time to trilineage engraftment was unchanged compared with historical controls. We conclude that pretreatment with rhG-CSF prior to marrow harvest may improve the graft by increasing the total number of myeloid lineage restricted progenitor cells, resulting in stable but not accelerated myeloid engraftment of autologous marrow.
Authors:
H E Johnsen; P B Hansen; T Plesner; L Jensen; E Gaarsdal; H Andersen; S W Hansen; H Birgens; G K Jacobsen; E Kjaersgaard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bone marrow transplantation     Volume:  10     ISSN:  0268-3369     ISO Abbreviation:  Bone Marrow Transplant.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-12-04     Completed Date:  1992-12-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8702459     Medline TA:  Bone Marrow Transplant     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  229-34     Citation Subset:  IM    
Affiliation:
Department of Medicine and Haematology C, Herlev Hospital, University of Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow Transplantation / methods*
Cell Division / drug effects
Colony-Forming Units Assay
Evaluation Studies as Topic
Granulocyte Colony-Stimulating Factor / administration & dosage,  adverse effects,  therapeutic use*
Hematopoiesis / drug effects
Hematopoietic Stem Cell Transplantation*
Hematopoietic Stem Cells / cytology,  drug effects*
Humans
Male
Neoplasms / surgery
Phenotype
Safety
Transplantation, Autologous
Chemical
Reg. No./Substance:
143011-72-7/Granulocyte Colony-Stimulating Factor
Comments/Corrections
Comment In:
Bone Marrow Transplant. 1993 Oct;12(4):422   [PMID:  7506092 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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