Document Detail

Increased viable osteoblast density in the presence of nanophase compared to conventional alumina and titania particles.
MedLine Citation:
PMID:  15046907     Owner:  NLM     Status:  MEDLINE    
In the present in vitro study, osteoblast (bone-forming cells) viability and cell density were investigated when cultured in the presence of nanophase compared to conventional (i.e. micron) alumina and titania particles at various concentrations (from 10,000 to 100 microg/ml of cell culture media) for up to 6h. Results confirmed previous studies of the detrimental influences of all ceramic particulates on osteoblast viability and cell densities. For the first time, however, results provided evidence of increased apoptotic cell death when cultured in the presence of conventional compared to nanophase alumina and titania particles. Moreover, since material characterization studies revealed that the only difference between respective ceramic particles was nanometer- and conventional-dimensions (specifically, phase and chemical properties were similar between respective nanophase and conventional alumina as well as titania particles), these results indicated that osteoblast viability and densities were influenced solely by particle size. Such nanometer particulate wear debris may result from friction between articulating components of orthopedic implants composed of novel nanophase ceramic materials. Results of a less detrimental effect of nanometer--as compared to conventional-dimensioned particles on the functions of osteoblasts provide additional evidence that nanophase ceramics may become the next generation of bone prosthetic materials with increased efficacy and, thus, deserve further testing.
Luke G Gutwein; Thomas J Webster
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  25     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-03-29     Completed Date:  2004-12-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  4175-83     Citation Subset:  IM    
Department of Biomedical Engineering, Purdue University, 1296 Potter Building, West Lafayette, IN 47907-1296, USA.
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MeSH Terms
Aluminum Oxide / chemistry*
Cell Count
Cell Division / physiology
Cell Survival / physiology
Cells, Cultured
Nanotechnology / methods
Nanotubes / chemistry*,  ultrastructure*
Osseointegration / physiology
Osteoblasts / cytology*,  physiology*
Particle Size
Phase Transition
Tissue Engineering / methods*
Titanium / chemistry*
Reg. No./Substance:
0/Powders; 1344-28-1/Aluminum Oxide; 13463-67-7/titanium dioxide; 7440-32-6/Titanium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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