Document Detail


Increased tissue neutral endopeptidase 24.11 activity in spontaneously hypertensive hamsters.
MedLine Citation:
PMID:  9633795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to determine whether tissue neutral endopeptidase (NEP) 24.11 activity, a membrane-bound metalloenzyme widely distributed in the peripheral circulation that cleaves and inactivates vasodilator peptides, is increased in spontaneously hypertensive hamsters relative to genetically/age-matched normotensive hamsters. Mean arterial pressure and heart rate were 163 +/- 11 mm Hg and 312 +/- 7 beats/min in spontaneously hypertensive hamsters and 99 +/- 3 mm Hg and 302 +/- 10 beats/min in normotensive hamsters, respectively (mean +/- SEM). NEP 24.11 activity is significantly increased in the kidney, cheek pouch, and spinotrapezius muscle, and significantly decreased in the heart and aorta of spontaneously hypertensive hamsters relative to controls (P < .05). Lung and brain NEP 24.11 activity is similar in both groups. Renal NEP 24.11 activity increases and to a similar extent in spontaneously hypertensive and normotensive hamsters as chloride anion concentration in the assay buffer is increased. Substituting citrate for chloride anion significantly attenuates renal NEP 24.11 activity. Taken together, these data indicate that NEP 24.11 activity in spontaneously hypertensive hamsters is increased in two organs that contribute appreciably to peripheral vascular resistance, skeletal muscle, and kidney. We suggest that the spontaneously hypertensive hamster is a suitable model to study the role of skeletal muscle and renal NEP 24.11 in regulating vasomotor tone in essential hypertension.
Authors:
J K Vishwanatha; R G Davis; S Blumberg; X P Gao; I Rubinstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of hypertension     Volume:  11     ISSN:  0895-7061     ISO Abbreviation:  Am. J. Hypertens.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-08-24     Completed Date:  1998-08-24     Revised Date:  2009-02-24    
Medline Journal Info:
Nlm Unique ID:  8803676     Medline TA:  Am J Hypertens     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  585-90     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anions / pharmacology
Chlorides / pharmacology
Cricetinae / genetics
Glycopeptides / pharmacology
Hypertension / enzymology*,  genetics*
Kidney / drug effects,  enzymology
Neprilysin / metabolism*
Osmolar Concentration
Protease Inhibitors / pharmacology
Thiorphan / pharmacology
Grant Support
ID/Acronym/Agency:
DE00386/DE/NIDCR NIH HHS; DE10347/DE/NIDCR NIH HHS; GM46459/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Anions; 0/Chlorides; 0/Glycopeptides; 0/Protease Inhibitors; 36357-77-4/phosphoramidon; 76721-89-6/Thiorphan; EC 3.4.24.11/Neprilysin

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