| Increased thymidylate synthase (EC 2.1.1.45) activity in normal and neoplastic proliferation. | |
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MedLine Citation:
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PMID: 3224329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thymidylate (dTMP) synthase (EC 2.1.1.45) activity was measured in 100,000 x g supernatant fluid with a sensitive, rapid radio assay. The activity in normal rat liver was low (0.098-0.204 nmol/hr/mg protein). dTMP synthase specific activities in rat thymus, spleen, bone marrow, testis, lung, heart, brain, kidney, and small intestine were 6297, 1842, 1500, 788, 215, 76, 61, 39 and 24%, respectively, of that of the liver. The activity in 5-day-old rat liver was 16-fold higher than in adult. dTMP synthase activity increased in rat hepatomas to 7- to 125-fold of that of normal rat liver. There was a significant correlation between the increase in synthase activity and the proliferation rates of the hepatomas. In 8 human colon carcinomas, dTMP synthase activity increased to 2.9- to 8-fold of that of normal human colon mucosa. In leukemic leukocytes from 3 leukemia patients, activity was 8- to 10-fold higher than in normal leukocytes. |
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Authors:
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Y Hashimoto; T Shiotani; J N Eble; J L Glover; G Weber |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer biochemistry biophysics Volume: 10 ISSN: 0305-7232 ISO Abbreviation: Cancer Biochem. Biophys. Publication Date: 1988 Jul |
Date Detail:
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Created Date: 1989-04-05 Completed Date: 1989-04-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7506524 Medline TA: Cancer Biochem Biophys Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Affiliation:
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Laboratory for Experimental Oncology, Indiana University School of Medicine, Indianapolis 46223. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging Animals Cell Transformation, Neoplastic* Colon / enzymology Colonic Neoplasms / enzymology Cytosol / enzymology Humans Intestinal Mucosa / enzymology Kinetics Leukemia / enzymology Leukocytes / enzymology Liver / enzymology*, growth & development Liver Neoplasms, Experimental / enzymology* Male Organ Specificity Rats Reference Values Thymidylate Synthase / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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CA-13526/CA/NCI NIH HHS; CA-42510/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 2.1.1.45/Thymidylate Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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