Document Detail


Increased testicular 8-hydroxy-2'-deoxyguanosine (8-OHdG) and inducible nitric oxide synthetase (iNOS) and nuclear factor κB (NF-κB) expressions in experimental rat varicocele.
MedLine Citation:
PMID:  21341550     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To assess nuclear factor-kappaB (NF-kappaB), inducible NO synthase (iNOS) immunohistochemically, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) biochemically, which are sensitive biological markers of oxidative damage and stress, in testes with experimental varicocele.
MATERIALS AND METHODS: Adult rats were randomly divided into three groups. Control group (n: 10), sham group (n: 10), varicocele group (n: 10). Of 14 rats undergoing partial ligation of the left renal vein, 10 rats had developed dilation of the left spermatic vein when evaluated 3 months after varicocele-inducing surgery. The rats were sacrificed after 3 months of the varicocele-inducing surgery. Ipsilateral and contralateral testes were examined for 8-hydroxy-2'-deoxyguanosine (8-OHdG) biochemically, inducible NO synthase (iNOS) and nuclear factor-kappaB (NF-kappaB) expression immunohistochemically.
RESULTS: Inducible NO synthase (iNOS), nuclear factor-kappaB (NF-kappaB) expressions and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in both testes of varicocele group were markedly higher compared with control and sham groups (p < 0.01). There was no difference between control and sham groups (p > 0.05).
CONCLUSIONS: Regarding to our results, we suggest that varicocele may produce oxidative stress in both of testes, and we believe that this stress may play a role in male fertility.
Authors:
Volkan Tuğcu; Asuman Gedikbaşi; Bircan Mutlu; Ekrem Güner; Mehmet Uhri; Gülnur Andican; Emin Ozbek; Ali I Taşçi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archivio italiano di urologia, andrologia : organo ufficiale [di] Società italiana di ecografia urologica e nefrologica / Associazione ricerche in urologia     Volume:  82     ISSN:  1124-3562     ISO Abbreviation:  Arch Ital Urol Androl     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-02-23     Completed Date:  2011-03-31     Revised Date:  2011-05-12    
Medline Journal Info:
Nlm Unique ID:  9308247     Medline TA:  Arch Ital Urol Androl     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  148-53     Citation Subset:  IM    
Affiliation:
Bakirköy Dr. Sadi Konuk Training and Research Hospital, Department of Urology, Istanbul, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Animals
Deoxyguanosine / analogs & derivatives*,  biosynthesis
Male
NF-kappa B / biosynthesis*
Nitric Oxide Synthase Type II / biosynthesis*
Rats
Rats, Sprague-Dawley
Testis / enzymology,  metabolism*
Up-Regulation / physiology*
Varicocele / enzymology,  metabolism*
Chemical
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/NF-kappa B; 961-07-9/Deoxyguanosine; EC 1.14.13.39/Nitric Oxide Synthase Type II

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