| Increased synthesis of α-tocopherol, paramylon and tyrosine by Euglena gracilis under conditions of high biomass production. | |
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MedLine Citation:
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PMID: 20854454 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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AIMS: To analyse the production of different metabolites by dark-grown Euglena gracilis under conditions found to render high cell growth. METHODS AND RESULTS: The combination of glutamate (5 g l(-1) ), malate (2 g l(-1) ) and ethanol (10 ml l(-1) ) (GM + EtOH); glutamate (7·15 g l(-1) ) and ethanol (10 ml l(-1) ); or malate (8·16 g l(-1) ), glucose (10·6 g l(-1) ) and NH(4) Cl (1·8 g l(-1) ) as carbon and nitrogen sources, promoted an increase of 5·6, 3·7 and 2·6-fold, respectively, in biomass concentration in comparison with glutamate and malate (GM). In turn, the production of α-tocopherol after 120 h identified by LC-MS was 3·7 ± 0·2, 2·4 ± 0·1 and 2 ± 0·1 mg [g dry weight (DW)](-1) , respectively, while in the control medium (GM) it was 0·72 ± 0·1 mg (g DW)(-1) . For paramylon synthesis, the addition of EtOH or glucose induced a higher production. Amino acids were assayed by RP-HPLC; Tyr a tocopherol precursor and Ala an amino acid with antioxidant activity were the amino acids synthesized at higher concentration. CONCLUSIONS: Dark-grown E. gracilis Z is a suitable source for the generation of the biotechnologically relevant metabolites tyrosine, α-tocopherol and paramylon. SIGNIFICANCE AND IMPACT OF THE STUDY: By combining different carbon and nitrogen sources and inducing a tolerable stress to the cell by adding ethanol, it was possible to increase the production of biomass, paramylon, α-tocopherol and some amino acids. The concentrations of α-tocopherol achieved in this study are higher than others reported previously for Euglena, plant and algal systems. This work helps to understand the effect of different carbon sources on the synthesis of bio-molecules by E. gracilis and can be used as a basis for future works to improve the production of different metabolites of biotechnological importance by this organism. |
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Authors:
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J S Rodríguez-Zavala; M A Ortiz-Cruz; G Mendoza-Hernández; R Moreno-Sánchez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-21 |
Journal Detail:
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Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 2160-72 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology. |
Affiliation:
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Departamento de Bioquímica, Instituto Nacional de Cardiología, México D.F., México. rodjos@cardiologia.org.mx |
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