Document Detail


Increased susceptibility to liver damage from pneumoperitoneum in a murine model of biliary atresia.
MedLine Citation:
PMID:  20850622     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HYPOTHESIS: We hypothesized that livers with biliary atresia (BA) are more susceptible to the harmful effects of a high-pressure CO(2) pneumoperitoneum (PP) than healthy livers.
METHODS: A murine model of BA was used in this experiment. Mice were divided into 6 groups: (1) control Balb/c; (2) control Balb/c, CO(2)-PP; (3) control BA; (4) BA-sham; (5) BA, CO(2)-PP; and (6) BA, air-PP. Mice from groups 2, 5, and 6 underwent an 8-mm Hg-PP for 60 minutes. Liver samples were collected for histology, colorimetry, and flow cytometry analysis 18 to 24 hours after the procedure. Markers of apoptosis were investigated as indicators of acute cell damage.
RESULTS: We observed a statistically significant higher rate of apoptosis in livers with BA exposed to a prolonged CO(2)-PP or air-PP compared with control groups. There were no significant differences between groups 1 and 2, or between groups 5 and 6.
CONCLUSIONS: In this animal model, we have shown that livers with BA are more susceptible than healthy livers to injury by a prolonged PP. This injury was caused by both CO(2) and air-PP, implying that it is the direct result of pressure. These results may have implications for the success of minimally invasive Kasai procedures.
Authors:
Pablo Laje; Fred H Clark; Joshua R Friedman; Alan W Flake
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  45     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2011-02-03     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1791-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biliary Atresia / complications,  surgery*
Disease Models, Animal
Disease Susceptibility
Liver Failure / etiology*
Mice
Mice, Inbred BALB C
Pneumoperitoneum, Artificial / adverse effects*
Grant Support
ID/Acronym/Agency:
R01 DK079881/DK/NIDDK NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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