Document Detail

Increased stability of Bcl-2 in HSP70-mediated protection against apoptosis induced by oxidative stress.
MedLine Citation:
PMID:  20890773     Owner:  NLM     Status:  MEDLINE    
We have previously shown that heat shock protein 70 (HSP70) markedly inhibits H(2)O(2)-induced apoptosis in mouse C2C12 myogenic cells by reducing the release of Smac. However, the molecular mechanism by which HSP70 interferes with Smac release during oxidative stress-induced apoptosis is not understood. In the current study, we showed that HSP70 increased the stability of Bcl-2 during oxidative stress. An antisense phosphorothioate oligonucleotide against Bcl-2 caused selective inhibition of Bcl-2 protein expression induced by HSP70 and significantly attenuated HSP70-mediated cell protection against H(2)O(2)-induced release of Smac and apoptosis. Taken together, our results indicate that there are important relationships among HSP70, Bcl-2, release of Smac, and induction of apoptosis by oxidative stress.
Bimei Jiang; Pengfei Liang; Gonghua Deng; Zizhi Tu; Meidong Liu; Xianzhong Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-03
Journal Detail:
Title:  Cell stress & chaperones     Volume:  16     ISSN:  1466-1268     ISO Abbreviation:  Cell Stress Chaperones     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-01-28     Completed Date:  2011-06-15     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9610925     Medline TA:  Cell Stress Chaperones     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  143-52     Citation Subset:  IM    
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China.
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MeSH Terms
Blotting, Western
Caspases / metabolism
Cell Survival / drug effects
Cells, Cultured
Enzyme Activation / drug effects
Flow Cytometry
Gene Expression Regulation / drug effects
HSP70 Heat-Shock Proteins / genetics,  metabolism*
Oligonucleotides, Antisense / pharmacology
Oxidative Stress*
Protein Stability
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Oligonucleotides, Antisense; 0/Proto-Oncogene Proteins c-bcl-2; EC 3.4.22.-/Caspases

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