| Increased stability of Bcl-2 in HSP70-mediated protection against apoptosis induced by oxidative stress. | |
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MedLine Citation:
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PMID: 20890773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that heat shock protein 70 (HSP70) markedly inhibits H(2)O(2)-induced apoptosis in mouse C2C12 myogenic cells by reducing the release of Smac. However, the molecular mechanism by which HSP70 interferes with Smac release during oxidative stress-induced apoptosis is not understood. In the current study, we showed that HSP70 increased the stability of Bcl-2 during oxidative stress. An antisense phosphorothioate oligonucleotide against Bcl-2 caused selective inhibition of Bcl-2 protein expression induced by HSP70 and significantly attenuated HSP70-mediated cell protection against H(2)O(2)-induced release of Smac and apoptosis. Taken together, our results indicate that there are important relationships among HSP70, Bcl-2, release of Smac, and induction of apoptosis by oxidative stress. |
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Authors:
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Bimei Jiang; Pengfei Liang; Gonghua Deng; Zizhi Tu; Meidong Liu; Xianzhong Xiao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-03 |
Journal Detail:
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Title: Cell stress & chaperones Volume: 16 ISSN: 1466-1268 ISO Abbreviation: Cell Stress Chaperones Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-01-28 Completed Date: 2011-06-15 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 9610925 Medline TA: Cell Stress Chaperones Country: Netherlands |
Other Details:
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Languages: eng Pagination: 143-52 Citation Subset: IM |
Affiliation:
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Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Blotting, Western Caspases / metabolism Cell Survival / drug effects Cells, Cultured Enzyme Activation / drug effects Flow Cytometry Gene Expression Regulation / drug effects HSP70 Heat-Shock Proteins / genetics, metabolism* Mice Oligonucleotides, Antisense / pharmacology Oxidative Stress* Protein Stability Proto-Oncogene Proteins c-bcl-2 / metabolism* Transfection |
| Chemical | |
Reg. No./Substance:
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0/HSP70 Heat-Shock Proteins; 0/Oligonucleotides, Antisense; 0/Proto-Oncogene Proteins c-bcl-2; EC 3.4.22.-/Caspases |
| Comments/Corrections | |
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