| Increased sinusoidal volume and solute extraction during retrograde liver perfusion. | |
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MedLine Citation:
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PMID: 2735410 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retrograde isolated liver perfusion has been used to probe acinar functional heterogeneity, but the hemodynamic effects of backward flow have not been characterized. In this study, extraction of a long-chain fatty acid derivative, 12-N-methyl-7-nitrobenzo-2-oxa-1,3-diazol-amino stearate (12-NBDS), was greater during retrograde than during anterograde perfusion of isolated rat liver. To determine whether hemodynamic differences between anterograde and retrograde perfused livers could account for this finding, the hepatic extracellular space was measured for both directions of flow by means of [14C]sucrose washout during perfusion as well as by direct measurement of [14C]sucrose entrapped during perfusion. A three- to fourfold enlargement of the total hepatic extracellular space was found during retrograde perfusion by both approaches. Examination of perfusion-fixed livers by light microscopy and morphometry revealed that marked distension of the sinusoids occurred during retrograde perfusion and that this accounts for the observed increase in the [14C]sucrose space. These findings support the hypothesis that maximum resistance to perfusate flow in the isolated perfused rat liver is located at the presinusoidal level. In addition, increased transit time of perfusate through the liver and greater sinusoidal surface area resulting from sinusoidal distension may account for the higher extraction of 12-NBDS and possibly other compounds by retrograde perfused liver. |
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Authors:
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N M Bass; J A Manning; R A Weisiger |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 256 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1989 Jun |
Date Detail:
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Created Date: 1989-07-18 Completed Date: 1989-07-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G1041-8 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of California, San Francisco 94143. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4-Chloro-7-nitrobenzofurazan
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analogs & derivatives,
metabolism Animals Carbon Radioisotopes Kinetics Liver / cytology, physiology* Liver Circulation* Male Perfusion Rats Rats, Inbred Strains Stearates / metabolism Sucrose / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AM-26743/AM/NIADDK NIH HHS; DK-32898/DK/NIDDK NIH HHS; DK-32926/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Stearates; 10199-89-0/4-Chloro-7-nitrobenzofurazan; 117056-67-4/12-N-methyl-7-nitrobenzo-2-oxa-1,3-diazolamino stearate; 57-50-1/Sucrose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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