Document Detail


Increased severity of acute cerebral ischemic injury correlates with enhanced stem cell induction as well as with predictive behavioral profiling.
MedLine Citation:
PMID:  16181088     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An 8-vessel-occlusion (8VO) method was developed to compare with the conventional 4-vessel-occlusion (4VO) in hippocampal ischemic damage and progenitor cell induction 10 days following ischemia in female rats. Eight posture-relevant tests were performed following ischemia to correlate grades of postural abnormality with the histological outcome. The total hippocampal living cell ratio including 7 hippocampal subregions in 8VO group (n=11) was much lower than that in 4VO group (n=10, 51+/-5% vs. 78+/-4, p<0.01). In 4VO group, BrdU positive cells were mainly located in the subgranular zone (SGZ) with a count of 54+/-20/mm2 (7micro-thick slice), comparable to the maximal level following global ischemia in male gerbils and rats reported so far referring to slice-thickness differences (50-60 micro-thick slices). Similarly, nestin-bearing cells were 29+/-11/ mm2. In 8VO group, BrdU and nestin positive cells increased by 10 times. Triple staining of BrdU, nestin and DAPI demonstrated that BrdU-immunoreactivity was extensively distributed in the hippocampal hilus while the nestin was mainly located along the SGZ. Most of nestin labeling was not co-localized with the BrdU, indicating that establishment of these cells might precede BrdU injections (8 and 9d post ischemia). Behavioral scores were much greater for 8VO group than for 4VO group and composite postural scores well correlated with the hippocampal cell loss. In conclusion, severe ischemia correlates with vigorous induction of the hippocampal progenitor cells in rats while behavioral profiling of posture changes permits prediction of severity of damage.
Authors:
Zhen He; Li Cui; Samuel S Wu; Xiao-Yong Li; James W Simpkins; Michael McKinney; Arthur L Day
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Current neurovascular research     Volume:  1     ISSN:  1567-2026     ISO Abbreviation:  -     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-09-26     Completed Date:  2005-11-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101208439     Medline TA:  Curr Neurovasc Res     Country:  United Arab Emirates    
Other Details:
Languages:  eng     Pagination:  399-409     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Mayo Clinic Jacksonville, 4500 San Pablo Road, Jacksonville, FL 32224, USA. he.zhen@mayo.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal / physiology*
Bromodeoxyuridine / metabolism
Cell Count / methods
Disease Models, Animal
Female
Hippocampus / pathology
Immunohistochemistry / methods
Indoles / diagnostic use
Intermediate Filament Proteins / metabolism
Ischemic Attack, Transient / pathology*,  physiopathology*
Nerve Tissue Proteins / metabolism
Neurons / pathology,  physiology
Posture / physiology
Predictive Value of Tests
Rats
Rats, Sprague-Dawley
Stem Cells / physiology*
Time Factors
Grant Support
ID/Acronym/Agency:
AG 10485/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Indoles; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/nestin; 47165-04-8/DAPI; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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