Document Detail


Increased resistance to myocardial ischemia in the Brown Norway vs. Dahl S rat: role of nitric oxide synthase and Hsp90.
MedLine Citation:
PMID:  15808839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hearts from Brown Norway (BN/Mcw) rats are more resistant to ischemia than hearts from Dahl S (SS/Mcw) rats. We determined whether nitric oxide (.NO) is responsible for increased cardioprotection in BN/Mcw vs. SS/Mcw hearts. Hearts from the two strains were treated with N(G)-monomethyl-L-arginine (L-NMA) or S-nitrosoglutathione (GSNO) before ischemia and reperfusion. Infarct size in untreated BN/Mcw hearts was approximately 63% less than in SS/Mcw hearts. Inhibiting NOS with L-NMA increased infarct size in BN/Mcw hearts to that observed in untreated SS/Mcw hearts but did not further increase injury in SS/Mcw hearts. The .NO donor GSNO decreased infarct size in SS/Mcw rats but had no effect on BN/Mcw hearts. Plasma and heart tissue from BN/Mcw rats contained 80% and 130% more nitrite + nitrate than that from SS/Mcw rats. These data suggest that increased .NO production protects BN/Mcw hearts from ischemic injury. Real time PCR showed no differences in NOS1, NOS2 or NOS3 isozyme transcripts in the hearts from the two strains. NOS3 was the only isozyme detected by western analysis. Both strains exhibited the same level of NOS3 and hsp90 protein expression. However, hsp90 association with NOS3 in BN/Mcw hearts was increased twofold compared with SS/Mcw hearts. Inhibiting hsp90-NOS3 interaction with geldanamycin decreased the resistance to ischemia in BN/Mcw hearts but not in SS/Mcw hearts. SS/Mcw hearts also generated three times more N(omega)-nitro-L-arginine-methylester inhibitable superoxide than BN/Mcw hearts. These findings indicate that hsp90 with NOS3 increases .NO production and decreases uncoupled NOS3 activity. We conclude increased association of hsp90 with NOS3 is a major mechanism by which BN/Mcw hearts are more resistant to ischemia than SS/Mcw hearts.
Authors:
Yang Shi; William Hutchins; Hitoshi Ogawa; Chung-Che Chang; Kirkwood A Pritchard; Chenyang Zhang; Pawjai Khampang; Jozef Lazar; Howard J Jacob; Parvaneh Rafiee; John E Baker
Related Documents :
9572119 - Cardiac looping--an uneasy deal with laterality.
664119 - The inherent mathematical error factors in the radiological determination of heart volume.
10657379 - Sodium pump isoforms in xenotransplantation: importance of biochemical compatibility.
7373269 - Electrical activity in cardiac mutant axolotl hearts.
5057019 - Healing over and muscle contraction in toad hearts.
10768239 - Vertebral scale system to measure heart size in radiographs.
2355459 - Familial dilated cardiomyopathy complicated by left ventricular aneurysm.
2527749 - Myocardial hypertrophy and left ventricular diastolic function in hypertensive patients...
21720779 - Impact of chronic kidney disease and stress myocardial perfusion imaging as a predictor...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  38     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-05     Completed Date:  2005-09-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  625-35     Citation Subset:  IM    
Affiliation:
Division of Pediatric Surgery, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. yangshi@mcw.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Enzyme Inhibitors / pharmacology
HSP90 Heat-Shock Proteins / metabolism*
Myocardial Ischemia / enzymology*,  metabolism
Nerve Tissue Proteins / antagonists & inhibitors,  genetics,  physiology
Nitrates / metabolism
Nitric Oxide / metabolism*
Nitric Oxide Synthase / antagonists & inhibitors,  genetics,  physiology*
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Nitrites / metabolism
RNA, Messenger / analysis,  metabolism
Rats
Rats, Inbred BN
Rats, Inbred Dahl
Species Specificity
Superoxides / metabolism
omega-N-Methylarginine / pharmacology
Grant Support
ID/Acronym/Agency:
HL61417/HL/NHLBI NIH HHS; HL65203/HL/NHLBI NIH HHS; HL66334/HL/NHLBI NIH HHS; HL71214/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/HSP90 Heat-Shock Proteins; 0/Nerve Tissue Proteins; 0/Nitrates; 0/Nitrites; 0/RNA, Messenger; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; 17035-90-4/omega-N-Methylarginine; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos1 protein, rat; EC 1.14.13.39/Nos2 protein, rat; EC 1.14.13.39/Nos3 protein, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Role of p38alpha MAPK in cardiac apoptosis and remodeling after myocardial infarction.
Next Document:  Interleukin-1beta attenuates beta-very low-density lipoprotein uptake and its receptor expression in...