| Increased TIMP/MMP ratio in varicose veins: a possible explanation for extracellular matrix accumulation. | |
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MedLine Citation:
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PMID: 10951407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Primary varicose veins are functionally characterized by venous back-flow and blood stagnation in the upright position. Dilatation and tortuosity provide evidence for progressive venous wall remodelling, with disturbance of smooth muscle cell/extracellular matrix organization. Affected areas are not uniformly distributed, some areas being hypertrophic, whereas others are atrophic or unaffected. In 12 varicose veins and ten control veins, the proteolytic enzyme/inhibitor balance which may participate in the remodelling of the venous wall was investigated. For this purpose, the presence and enzymatic activity of matrix metalloproteinases (MMP-2, MMP-9), tissue inhibitors of MMPs (TIMP-1, TIMP-2), urokinase-type (uPA) and tissue-type (tPA) plasminogen activators (PAs), and plasminogen activator inhibitor-1 (PAI-1) were quantified by western blot and gelatin or plasminogen-casein zymography. In addition, MMP-2, TIMP-1, TIMP-2, and PAI-1 levels were measured by ELISA. A high TIMP-1 level and a low MMP-2 level/activity were found in varicose veins (p<0.005), resulting in a three-fold increase in the TIMP-1/MMP-2 ratio in varicose versus control veins. Levels of PAs (uPA and tPA) as well as PAI-1 were both lower in varicose veins (p<0.005), with minimal change in the PAI/PA ratio. These results demonstrate that varicose veins are characterized by a higher than normal TIMP/MMP ratio, which may facilitate extracellular matrix accumulation in the diseased venous wall. |
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Authors:
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C Badier-Commander; T Verbeuren; C Lebard; J B Michel; M P Jacob |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pathology Volume: 192 ISSN: 0022-3417 ISO Abbreviation: J. Pathol. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-10-24 Completed Date: 2000-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0204634 Medline TA: J Pathol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 105-12 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Ltd. |
Affiliation:
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U460 INSERM, UFR de Médecine X. Bichat, 16 Rue H. Huchard, 75870 Paris Cedex 18, France. badier@bichat.inserm.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blotting, Western Extracellular Matrix / enzymology, metabolism* Female Humans Male Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism Matrix Metalloproteinases / metabolism* Middle Aged Plasminogen Activators / metabolism Plasminogen Inactivators / metabolism Tissue Inhibitor of Metalloproteinase-1 / metabolism Tissue Inhibitor of Metalloproteinase-2 / metabolism Tissue Inhibitor of Metalloproteinases / metabolism* Varicose Veins / enzymology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Plasminogen Inactivators; 0/Tissue Inhibitor of Metalloproteinase-1; 0/Tissue Inhibitor of Metalloproteinases; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 3.4.21.-/Plasminogen Activators; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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