Document Detail

Increased rates of fatty acid uptake and plasmalemmal fatty acid transporters in obese Zucker rats.
MedLine Citation:
PMID:  11504711     Owner:  NLM     Status:  MEDLINE    
Giant vesicles were used to study the rates of uptake of long-chain fatty acids by heart, skeletal muscle, and adipose tissue of obese and lean Zucker rats. With obesity there was an increase in vesicular fatty acid uptake of 1.8-fold in heart, muscle and adipose tissue. In some tissues only fatty acid translocase (FAT) mRNA (heart, +37%; adipose, +80%) and fatty acid-binding protein (FABPpm) mRNA (heart, +148%; adipose, +196%) were increased. At the protein level FABPpm expression was not changed in any tissues except muscle (+14%), and FAT/CD36 protein content was altered slightly in adipose tissue (+26%). In marked contrast, the plasma membrane FAT/CD36 protein was increased in heart (+60%), muscle (+80%), and adipose tissue (+50%). The plasma membrane FABPpm was altered only in heart (+50%) and adipose tissues (+70%). Thus, in obesity, alterations in fatty acid transport in metabolically important tissues are not associated with changes in fatty acid transporter mRNAs or altered fatty acid transport protein expression but with their increased abundance at the plasma membrane. We speculate that in obesity fatty acid transporters are relocated from an intracellular pool to the plasma membrane in heart, muscle, and adipose tissues.
J J Luiken; Y Arumugam; D J Dyck; R C Bell; M M Pelsers; L P Turcotte; N N Tandon; J F Glatz; A Bonen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2001-08-14
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  276     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-29     Completed Date:  2001-12-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  40567-73     Citation Subset:  IM    
Department of Kinesiology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
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MeSH Terms
Antigens, CD36
Fatty Acids / metabolism*
Membrane Glycoproteins / genetics,  metabolism*
Obesity / metabolism*
Organic Anion Transporters / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Rats, Zucker
Grant Support
Reg. No./Substance:
0/Antigens, CD36; 0/Fatty Acids; 0/Membrane Glycoproteins; 0/Organic Anion Transporters; 0/RNA, Messenger

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