Document Detail

Increased proteolytic processing of full-length Gli2 transcription factor reduces the hedgehog pathway activity in vivo.
MedLine Citation:
PMID:  21337666     Owner:  NLM     Status:  MEDLINE    
The proteolytic processing of Gli2 and Gli3 full-length transcription factors into repressors is a key step of the regulation in Hedgehog (Hh) signaling. The differential Gli2 and Gli3 processing is controlled by the processing determinant domain or PDD, but its significance is not clear. We generated a Gli2 mutant allele, Gli2(3PDD) , in which the Gli3PDD substitutes for the Gli2PDD. As expected, Gli2(3PDD) is processed more efficiently and at a different position as compared to Gli2, indicating that PDD also determines the extent and site of Gli2 and Gli3 processing in vivo. The increase in levels of the Gli2 repressor in Gli2(3PDD) mutant reduces the Hh pathway activity. Gli2(3PDD) processing is still regulated by Hh signaling. These results indicate that the proper balance between the Gli2 full-length activator and repressor is essential for Hh signaling.
Juan Li; Chengbing Wang; Yong Pan; Zengliang Bai; Baolin Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-02-18
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  240     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-15     Completed Date:  2011-07-11     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  766-74     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
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MeSH Terms
Cells, Cultured
Down-Regulation / genetics
Embryo, Mammalian
Gene Expression Regulation, Developmental
Hedgehog Proteins / metabolism,  physiology*
Kruppel-Like Transcription Factors / chemistry,  genetics*,  metabolism*
Mice, Inbred C57BL
Mice, Transgenic
Protein Isoforms / chemistry,  genetics,  metabolism
Protein Processing, Post-Translational / genetics*
Protein Structure, Tertiary / genetics
Signal Transduction / genetics,  physiology
Transcription Factors / chemistry,  genetics,  metabolism
Grant Support
R01 CA111673/CA/NCI NIH HHS; R01 GM070820/GM/NIGMS NIH HHS; R01 GM070820-01/GM/NIGMS NIH HHS; R01 GM070820-02/GM/NIGMS NIH HHS; R01 GM070820-03/GM/NIGMS NIH HHS; R01 GM070820-04/GM/NIGMS NIH HHS; R01 GM070820-05/GM/NIGMS NIH HHS; R01 GM070820-06A1/GM/NIGMS NIH HHS; R01 GM070820-06A1S1/GM/NIGMS NIH HHS; R01 GM070820-07/GM/NIGMS NIH HHS; R01GM70820/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Gli2 protein; 0/Hedgehog Proteins; 0/Kruppel-Like Transcription Factors; 0/Protein Isoforms; 0/Transcription Factors

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