Document Detail


Increased proteolytic processing of full-length Gli2 transcription factor reduces the hedgehog pathway activity in vivo.
MedLine Citation:
PMID:  21337666     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The proteolytic processing of Gli2 and Gli3 full-length transcription factors into repressors is a key step of the regulation in Hedgehog (Hh) signaling. The differential Gli2 and Gli3 processing is controlled by the processing determinant domain or PDD, but its significance is not clear. We generated a Gli2 mutant allele, Gli2(3PDD) , in which the Gli3PDD substitutes for the Gli2PDD. As expected, Gli2(3PDD) is processed more efficiently and at a different position as compared to Gli2, indicating that PDD also determines the extent and site of Gli2 and Gli3 processing in vivo. The increase in levels of the Gli2 repressor in Gli2(3PDD) mutant reduces the Hh pathway activity. Gli2(3PDD) processing is still regulated by Hh signaling. These results indicate that the proper balance between the Gli2 full-length activator and repressor is essential for Hh signaling.
Authors:
Juan Li; Chengbing Wang; Yong Pan; Zengliang Bai; Baolin Wang
Related Documents :
20531406 - A human b-cell interactome identifies myb and foxm1 as master regulators of proliferati...
23413906 - Effect of immune modulation therapy on cardiac function and t-bet/gata-3 gene expressio...
23010746 - Calcitonin gene-related peptide is a promising marker in ulcerative colitis.
23164156 - Intrahepatic mrna levels of socs1 and socs3 are associated with cirrhosis but do not pr...
22829926 - Protein inhibitor of activated stat, piasy regulates α-smooth muscle actin expression b...
1846596 - Novel downstream elements upregulate transcription initiated from an epstein-barr virus...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-02-18
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  240     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-15     Completed Date:  2011-07-11     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  766-74     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Down-Regulation / genetics
Embryo, Mammalian
Female
Gene Expression Regulation, Developmental
Hedgehog Proteins / metabolism,  physiology*
Kruppel-Like Transcription Factors / chemistry,  genetics*,  metabolism*
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Protein Isoforms / chemistry,  genetics,  metabolism
Protein Processing, Post-Translational / genetics*
Protein Structure, Tertiary / genetics
Signal Transduction / genetics,  physiology
Transcription Factors / chemistry,  genetics,  metabolism
Up-Regulation
Grant Support
ID/Acronym/Agency:
R01 CA111673/CA/NCI NIH HHS; R01 GM070820/GM/NIGMS NIH HHS; R01 GM070820-01/GM/NIGMS NIH HHS; R01 GM070820-02/GM/NIGMS NIH HHS; R01 GM070820-03/GM/NIGMS NIH HHS; R01 GM070820-04/GM/NIGMS NIH HHS; R01 GM070820-05/GM/NIGMS NIH HHS; R01 GM070820-06A1/GM/NIGMS NIH HHS; R01 GM070820-06A1S1/GM/NIGMS NIH HHS; R01 GM070820-07/GM/NIGMS NIH HHS; R01GM70820/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Gli2 protein; 0/Hedgehog Proteins; 0/Kruppel-Like Transcription Factors; 0/Protein Isoforms; 0/Transcription Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evolution of vertebrate appendicular structures: Insight from genetic and palaeontological data.
Next Document:  Effect of one D-Leu residue on right-handed helical -L-Leu-Aib- peptides in the crystal state.